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UniProt functional annotation for O28440 | ||
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| UniProt code: O28440. |
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| Organism: | |
Archaeoglobus fulgidus (strain ATCC 49558 / VC-16 / DSM 4304 / JCM 9628 / NBRC 100126). |
| Taxonomy: | |
Archaea; Euryarchaeota; Archaeoglobi; Archaeoglobales; Archaeoglobaceae; Archaeoglobus. |
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| Function: | |
Specifically catalyzes the NAD or NADP-dependent dehydrogenation of L-aspartate to iminoaspartate. {ECO:0000255|HAMAP- Rule:MF_01265, ECO:0000269|PubMed:16731057}. |
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| Catalytic activity: | |
Reaction=H2O + L-aspartate + NADP(+) = H(+) + NADPH + NH4(+) + oxaloacetate; Xref=Rhea:RHEA:11784, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16452, ChEBI:CHEBI:28938, ChEBI:CHEBI:29991, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.4.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01265, ECO:0000269|PubMed:16731057}; |
| Catalytic activity: | |
Reaction=H2O + L-aspartate + NAD(+) = H(+) + NADH + NH4(+) + oxaloacetate; Xref=Rhea:RHEA:11788, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16452, ChEBI:CHEBI:28938, ChEBI:CHEBI:29991, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.4.1.21; Evidence={ECO:0000255|HAMAP-Rule:MF_01265, ECO:0000269|PubMed:16731057}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.19 mM for L-aspartate (in the presence of NAD) {ECO:0000269|PubMed:16731057}; KM=4.3 mM for L-aspartate (in the presence of NADP) {ECO:0000269|PubMed:16731057}; KM=0.11 mM for NAD {ECO:0000269|PubMed:16731057}; KM=0.32 mM for NADP {ECO:0000269|PubMed:16731057}; KM=1.2 mM for oxaloacetate (in the presence of NADH and NH(3)) {ECO:0000269|PubMed:16731057}; KM=0.014 mM for NADH (in the presence of oxaloacetate and NH(3)) {ECO:0000269|PubMed:16731057}; KM=167 mM for NH(3) (in the presence of oxaloacetate and NADH) {ECO:0000269|PubMed:16731057}; pH dependence: Optimum pH is around 11.6. {ECO:0000269|PubMed:16731057}; Temperature dependence: Optimum temperature is 80 degrees Celsius. {ECO:0000269|PubMed:16731057}; |
| Pathway: | |
Cofactor biosynthesis; NAD(+) biosynthesis; iminoaspartate from L-aspartate (dehydrogenase route): step 1/1. {ECO:0000255|HAMAP- Rule:MF_01265, ECO:0000305|PubMed:16731057}. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:16731057, ECO:0000269|PubMed:17651440}. |
| Miscellaneous: | |
The iminoaspartate product is unstable in aqueous solution and can decompose to oxaloacetate and ammonia. {ECO:0000255|HAMAP-Rule:MF_01265}. |
| Similarity: | |
Belongs to the L-aspartate dehydrogenase family. {ECO:0000255|HAMAP-Rule:MF_01265}. |
Annotations taken from UniProtKB at the EBI.