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PDBsum entry 2dbe
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References listed in PDB file
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Key reference
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Title
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Crystal structure of a berenil-Dodecanucleotide complex: the role of water in sequence-Specific ligand binding.
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Authors
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D.G.Brown,
M.R.Sanderson,
J.V.Skelly,
T.C.Jenkins,
T.Brown,
E.Garman,
D.I.Stuart,
S.Neidle.
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Ref.
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Embo J, 1990,
9,
1329-1334.
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PubMed id
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Abstract
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The three-dimensional structure of a complex between the dodecanucleotide
d(CGCGAATTCGCG) and the anti-trypanocidal drug berenil, has been determined to a
resolution of 2.5 A. The structure has been solved by molecular replacement and
refined to an R factor of 0.177. A total of 49 water molecules have been
located. The drug is bound at the 5'-AAT-3' region of the oligonucleotide. At
one end of the drug the amidinium group is in hydrogen-bonded contact with N3 of
the adenine base complementary to the thymine of the AAT. The other amidinium
group does not make direct interactions with the DNA. Instead, a water molecule
mediates between them. This is in hydrogen-bonded contact with an amidinium
nitrogen atom, N3 of the 5' end adenine base and the ring oxygen atom of an
adjacent deoxyribose. Molecular mechanics calculations have been performed on
this complex, with the drug at various positions along the sequence. These show
that the observed position is only 0.8 kcal/mol higher in energy than the best
position. It is suggested that there is a broad energy well in the AATT region
for this drug, and that water molecules as well as the neighbouring sequence,
will determine precise positioning. More general aspects of minor groove binding
are discussed.
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Headers
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