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PDBsum entry 2d6y
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Gene regulation
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PDB id
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2d6y
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References listed in PDB file
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Key reference
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Title
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Sco4008, A putative tetr transcriptional repressor from streptomyces coelicolor a3(2), Regulates transcription of sco4007 by multidrug recognition.
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Authors
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T.Hayashi,
Y.Tanaka,
N.Sakai,
U.Okada,
M.Yao,
N.Watanabe,
T.Tamura,
I.Tanaka.
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Ref.
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J Mol Biol, 2013,
425,
3289-3300.
[DOI no: ]
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PubMed id
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Abstract
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SCO4008 from Streptomyces coelicolor A3(2) is a member of the TetR family.
However, its precise function is not yet clear. In this study, the crystal
structure of SCO4008 was determined at a resolution of 2.3Å, and its
DNA-binding properties were analyzed. Crystal structure analysis showed that
SCO4008 forms an Ω-shaped homodimer in which the monomer is composed of an
N-terminal DNA-binding domain containing a helix-turn-helix and a C-terminal
dimerization and regulatory domain possessing a ligand-binding cavity. The
genomic systematic evolution of ligands by exponential enrichment and
electrophoretic mobility shift assay revealed that four SCO4008 dimers bind to
the two operator regions located between sco4008 and sco4007, a secondary
transporter belonging to the major facilitator superfamily. Ligand screening
analysis showed that SCO4008 recognizes a wide range of structurally dissimilar
cationic and hydrophobic compounds. These results suggested that SCO4008 is a
transcriptional repressor of sco4007 responsible for the multidrug resistance
system in S. coelicolor A3(2).
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