UniProt functional annotation for P32455



	UniProt functional annotation for P32455

UniProt code: P32455.

Organism: Homo sapiens (Human).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.

Function: Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promotes oxidative killing and delivers antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes. {ECO:0000269|PubMed:22106366}.

Catalytic activity: Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; Evidence={ECO:0000269|PubMed:10676968, ECO:0000269|PubMed:16511497};

Subunit: Homodimer; homodimerization occurs upon GTP-binding and is required for the second hydrolysis step from GDP to GMP. Heterodimer with other family members, including GBP2, GBP3, GBP4 and GBP5. Dimerization regulates subcellular location to membranous structures. {ECO:0000269|PubMed:10970849, ECO:0000269|PubMed:16511497, ECO:0000269|PubMed:21151871}.

Subcellular location: Cytoplasm {ECO:0000269|PubMed:21151871}. Golgi apparatus membrane; Lipid-anchor {ECO:0000269|PubMed:21151871}; Cytoplasmic side. Cell membrane {ECO:0000269|PubMed:21151871}. Secreted. Note=Secreted from endothelial cells in the cerebrospinal fluid, upon bacterial challenge and independently of IFNG induction. Golgi membrane localization requires isoprenylation and the presence of another IFNG-induced factor.

Induction: By IFNG during macrophage activation, and by TNF and IL1B. {ECO:0000269|PubMed:17266443}.

Ptm: Isoprenylation is required for proper subcellular location. {ECO:0000269|PubMed:21151871}.

Similarity: Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. GB1 subfamily. {ECO:0000255|PROSITE-ProRule:PRU01052}.

Annotations taken from UniProtKB at the EBI.


Organism:		Homo sapiens (Human).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo.



Function:		Hydrolyzes GTP to GMP in 2 consecutive cleavage reactions. Exhibits antiviral activity against influenza virus. Promotes oxidative killing and delivers antimicrobial peptides to autophagolysosomes, providing broad host protection against different pathogen classes. {ECO:0000269\|PubMed:22106366}.


Catalytic activity:		Reaction=GTP + H2O = GDP + H(+) + phosphate; Xref=Rhea:RHEA:19669, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:37565, ChEBI:CHEBI:43474, ChEBI:CHEBI:58189; Evidence={ECO:0000269\|PubMed:10676968, ECO:0000269\|PubMed:16511497};
Subunit:		Homodimer; homodimerization occurs upon GTP-binding and is required for the second hydrolysis step from GDP to GMP. Heterodimer with other family members, including GBP2, GBP3, GBP4 and GBP5. Dimerization regulates subcellular location to membranous structures. {ECO:0000269\|PubMed:10970849, ECO:0000269\|PubMed:16511497, ECO:0000269\|PubMed:21151871}.
Subcellular location:		Cytoplasm {ECO:0000269\|PubMed:21151871}. Golgi apparatus membrane; Lipid-anchor {ECO:0000269\|PubMed:21151871}; Cytoplasmic side. Cell membrane {ECO:0000269\|PubMed:21151871}. Secreted. Note=Secreted from endothelial cells in the cerebrospinal fluid, upon bacterial challenge and independently of IFNG induction. Golgi membrane localization requires isoprenylation and the presence of another IFNG-induced factor.
Induction:		By IFNG during macrophage activation, and by TNF and IL1B. {ECO:0000269\|PubMed:17266443}.
Ptm:		Isoprenylation is required for proper subcellular location. {ECO:0000269\|PubMed:21151871}.
Similarity:		Belongs to the TRAFAC class dynamin-like GTPase superfamily. GB1/RHD3 GTPase family. GB1 subfamily. {ECO:0000255\|PROSITE-ProRule:PRU01052}.