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PDBsum entry 2cw7
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References listed in PDB file
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Key reference
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Title
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Crystal structure of intein homing endonuclease ii encoded in DNA polymerase gene from hyperthermophilic archaeon thermococcus kodakaraensis strain kod1.
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Authors
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H.Matsumura,
H.Takahashi,
T.Inoue,
T.Yamamoto,
H.Hashimoto,
M.Nishioka,
S.Fujiwara,
M.Takagi,
T.Imanaka,
Y.Kai.
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Ref.
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Proteins, 2006,
63,
711-715.
[DOI no: ]
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PubMed id
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Abstract
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No abstract given.
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Figure 1.
Figure 1. Structures of PI-TkoII, PI-PfuI, and PI-SceI. A:
Stereo ribbon diagram of PI-TkoII. PI-TkkoII contains the
endonuclease, Hint, III, and IV domains colored in green, blue,
light blue, and pink, respectively. Active site residues are
shown. B: Ribbon diagram of PI-PfuI. C: Ribbon diagram of
PI-SceI. D: Domain organization and multiple sequence alignment
of PI-TkoII, PI-PfuI, and PI-SceI. The aligned sequences were
displayed in ESPript.[24] Secondary structures were calculated
by DSSP.[25] Secondary structure annotations and numberings on
top correspond to PI-TkoII.  -Helices
are represented by spirals and  -strand
by arrows. White lettering boxed with a red background indicates
residues that are conserved in all three sequences, and red
lettering indicates similar residues. The conserved sequence
blocks of inteins are indicated.
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Figure 2.
Figure 2. A: Electrostatic charge distribution on the surface
of PI-TkoII; the view is projected in the same orientation as
Figure 1(A). B: View is rotated by 180° with respect to a
vertical axis. C: Docking model for the protein-DNA complex. The
minimal 16-bp DNA recognition sequence is shown as a ribbon.
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The above figures are
reprinted
by permission from John Wiley & Sons, Inc.:
Proteins
(2006,
63,
711-715)
copyright 2006.
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