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UniProt functional annotation for P9WPP9 | ||
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| UniProt code: P9WPP9. |
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| Organism: | |
Mycobacterium tuberculosis (strain ATCC 25618 / H37Rv). |
| Taxonomy: | |
Bacteria; Actinobacteria; Corynebacteriales; Mycobacteriaceae; Mycobacterium; Mycobacterium tuberculosis complex. |
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| Function: | |
Its precise biological substrate is not known. Catalyzes C14- demethylation of lanosterol, 24,25-dihydrolanosterol and obtusifoliol which is critical for ergosterol biosynthesis. It transforms lanosterol into 4,4'-dimethyl cholesta-8,14,24-triene-3-beta-ol. {ECO:0000269|PubMed:10430874, ECO:0000269|PubMed:9756611}. |
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| Catalytic activity: | |
Reaction=a 14alpha-methyl steroid + 3 O2 + 3 reduced [NADPH-- hemoprotein reductase] = a Delta(14) steroid + formate + 4 H(+) + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:54028, Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15740, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:138029, ChEBI:CHEBI:138031; EC=1.14.14.154; Evidence={ECO:0000269|PubMed:10430874}; |
| Catalytic activity: | |
Reaction=lanosterol + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = 4,4-dimethyl-5alpha-cholesta-8,14,24-trien-3beta-ol + formate + 4 H(+) + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:25286, Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15740, ChEBI:CHEBI:16521, ChEBI:CHEBI:17813, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210; Evidence={ECO:0000269|PubMed:10430874}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:25287; Evidence={ECO:0000269|PubMed:10430874}; |
| Catalytic activity: | |
Reaction=24,25-dihydrolanosterol + 3 O2 + 3 reduced [NADPH--hemoprotein reductase] = 4,4-dimethyl-8,14-cholestadien-3beta-ol + formate + 4 H(+) + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:45960, Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15740, ChEBI:CHEBI:28113, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210, ChEBI:CHEBI:78904; Evidence={ECO:0000269|PubMed:10430874}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:45961; Evidence={ECO:0000269|PubMed:10430874}; |
| Catalytic activity: | |
Reaction=3 O2 + obtusifoliol + 3 reduced [NADPH--hemoprotein reductase] = 4alpha-methyl-5alpha-ergosta-8,14,24(28)-trien-3beta-ol + formate + 4 H(+) + 4 H2O + 3 oxidized [NADPH--hemoprotein reductase]; Xref=Rhea:RHEA:14917, Rhea:RHEA-COMP:11964, Rhea:RHEA-COMP:11965, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:15740, ChEBI:CHEBI:17791, ChEBI:CHEBI:30109, ChEBI:CHEBI:57618, ChEBI:CHEBI:58210; Evidence={ECO:0000269|PubMed:10430874}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:14918; Evidence={ECO:0000269|PubMed:10430874}; |
| Cofactor: | |
Name=heme; Xref=ChEBI:CHEBI:30413; Evidence={ECO:0000269|PubMed:9756611}; |
| Activity regulation: | |
Inhibited by alpha-ethyl-N-4-pyridinyl- benzeneacetamide (EPBA) and 4,4'-dihydroxybenzophenone (DHBP). {ECO:0000269|PubMed:17846131}. |
| Pathway: | |
Steroid biosynthesis; zymosterol biosynthesis; zymosterol from lanosterol: step 1/6. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:11248033, ECO:0000269|PubMed:15530358, ECO:0000269|PubMed:17846131, ECO:0000269|PubMed:18367444, ECO:0000269|PubMed:19190730, ECO:0000269|Ref.8}. |
| Subcellular location: | |
Cytoplasm {ECO:0000305}. |
| Similarity: | |
Belongs to the cytochrome P450 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.