PDBsum entry 2cft

Phosphatase

PDB id: 2cft

Contents

Protein chain

293 a.a. *

Ligands

PLP

Metals

_CA ×2

Waters ×235

* Residue conservation analysis

PDB id:

2cft

Name:

Phosphatase

Title:

Crystal structure of human pyridoxal 5'-phosphate phosphatase with its substrate

Structure:

Pyridoxal phosphate phosphatase. Chain: a. Synonym: pyridoxal 5'-phosphate phosphatase, plp phosphatase. Engineered: yes

Source:

Homo sapiens. Human. Organism_taxid: 9606. Expressed in: escherichia coli. Expression_system_taxid: 511693.

Resolution:

1.80Å R-factor: 0.187 R-free: 0.226

Authors:

B.S.Kang,H.J.Cho,K.J.Kim,O.S.Kwon

Key ref:

B.S.Kang et al. Crystal structure of human pyridoxal 5-Phosphate phosphatase. To be published, .

Date:

23-Feb-06 Release date: 27-Feb-07

Protein chain

Q96GD0  (PLPP_HUMAN) -  Chronophin from Homo sapiens

Seq: 296 a.a.

Struc: 293 a.a.

Enzyme reactions

• Enzyme class 1: E.C.3.1.3.16 - protein-serine/threonine phosphatase.
• Reaction:
  1. O-phospho-L-seryl-[protein] + H2O = L-seryl-[protein] + phosphate
  2. O-phospho-L-threonyl-[protein] + H2O = L-threonyl-[protein] + phosphate
• Enzyme class 2: E.C.3.1.3.74 - pyridoxal phosphatase.
• Reaction: pyridoxal 5'-phosphate + H2O = pyridoxal + phosphate

pyridoxal 5'-phosphate (Bound ligand (Het Group name = PLP) corresponds exactly) + H2O = pyridoxal + phosphate

• Cofactor: Mg(2+)

Note, where more than one E.C. class is given (as above), each may correspond to a different protein domain or, in the case of polyprotein precursors, to a different mature protein.

Molecule diagrams generated from .mol files obtained from the KEGG ftp site