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UniProt functional annotation for P00915 | ||
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| UniProt code: P00915. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Reversible hydration of carbon dioxide. Can hydrates cyanamide to urea. {ECO:0000269|PubMed:10550681}. |
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| Catalytic activity: | |
Reaction=H(+) + hydrogencarbonate = CO2 + H2O; Xref=Rhea:RHEA:10748, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16526, ChEBI:CHEBI:17544; EC=4.2.1.1; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:12009884, ECO:0000269|PubMed:15299369, ECO:0000269|PubMed:16506782, ECO:0000269|PubMed:16870440, ECO:0000269|PubMed:17314045, ECO:0000269|PubMed:17407288, ECO:0000269|PubMed:6430186, ECO:0000269|PubMed:7932756, ECO:0000269|PubMed:804171, ECO:0000269|PubMed:8057362}; |
| Activity regulation: | |
Activated by histamine, imidazole, L-adrenaline, L- and D-histidine, and L- and D-phenylalanine. Inhibited by coumarins, sulfonamide derivatives such as acetazolamide, benzenesulfonamide and derivatives (4-carboxyethylbenzene-sulfonamide, 4-carboxyethylbenzene- sulfonamide ethyl ester, 4-(acetyl-2-aminoethyl)benzene-sulfonamide, 4- aminoethylbenzene-sulfonamide), and 'prong inhibitors' BR15, BR17, BR22 and BR30. Activated by a short exposition to Foscarnet (phosphonoformate trisodium salt), but inhibited by a long one. Esterase activity weakly reduced by cyanamide. {ECO:0000269|PubMed:16506782, ECO:0000269|PubMed:16686544, ECO:0000269|PubMed:16807956, ECO:0000269|PubMed:17127057, ECO:0000269|PubMed:17314045, ECO:0000269|PubMed:17407288, ECO:0000269|PubMed:18618712, ECO:0000269|PubMed:19186056, ECO:0000269|PubMed:19206230}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=4.0 mM for CO(2) {ECO:0000269|PubMed:10550681, ECO:0000269|PubMed:18618712}; KM=15 mM for 4-nitrophenyl acetate {ECO:0000269|PubMed:10550681, ECO:0000269|PubMed:18618712}; |
| Subcellular location: | |
Cytoplasm. |
| Similarity: | |
Belongs to the alpha-carbonic anhydrase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.