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UniProt functional annotation for P05102 | ||
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| UniProt code: P05102. |
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| Organism: | |
Haemophilus parahaemolyticus. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Pasteurellales; Pasteurellaceae; Haemophilus. |
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| Function: | |
This methylase recognizes the double-stranded sequence GCGC, causes specific methylation on C-2 on both strands, and protects the DNA from cleavage by the HhaI endonuclease. |
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| Catalytic activity: | |
Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5- methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine; Xref=Rhea:RHEA:13681, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:11370, ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789, ChEBI:CHEBI:85452, ChEBI:CHEBI:85454; EC=2.1.1.37; Evidence={ECO:0000255|PROSITE-ProRule:PRU10018}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=69 nM for DNA {ECO:0000269|PubMed:7899082}; KM=15 nM for S-adenosyl-L-methionine (SAM) {ECO:0000269|PubMed:7899082}; Vmax=87.0 nmol/min/mg enzyme {ECO:0000269|PubMed:7899082}; |
| Subunit: | |
Monomer. |
| Similarity: | |
Belongs to the class I-like SAM-binding methyltransferase superfamily. C5-methyltransferase family. {ECO:0000255|PROSITE- ProRule:PRU01016}. |
Annotations taken from UniProtKB at the EBI.