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UniProt functional annotation for Q8X1D8 | ||
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| UniProt code: Q8X1D8. |
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| Organism: | |
Fusarium oxysporum (Fusarium vascular wilt). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Sordariomycetes; Hypocreomycetidae; Hypocreales; Nectriaceae; Fusarium; Fusarium oxysporum species complex. |
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| Function: | |
Catalyzes the oxidative denitrification of neutral nitroalkanes, including 3-nitro-2-pentanol, 1-nitropropane, 2- nitropropane, nitroethane and nitrocyclohexane, and may thereby protect the organism against toxic compounds. Has no detectable acyl-CoA dehydrogenase activity. {ECO:0000269|PubMed:11867731, ECO:0000269|PubMed:16430210, ECO:0000269|PubMed:22538}. |
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| Catalytic activity: | |
Reaction=a primary nitroalkane + H2O + O2 = an aldehyde + H(+) + H2O2 + nitrite; Xref=Rhea:RHEA:20976, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:16301, ChEBI:CHEBI:17478, ChEBI:CHEBI:133972; EC=1.7.3.1; Evidence={ECO:0000269|PubMed:11867731, ECO:0000269|PubMed:12862464, ECO:0000269|PubMed:17994768, ECO:0000269|PubMed:19265437, ECO:0000269|PubMed:22538}; |
| Catalytic activity: | |
Reaction=a secondary nitroalkane + H2O + O2 = a ketone + H(+) + H2O2 + nitrite; Xref=Rhea:RHEA:26490, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16240, ChEBI:CHEBI:16301, ChEBI:CHEBI:17087, ChEBI:CHEBI:139218; EC=1.7.3.1; Evidence={ECO:0000269|PubMed:11867731, ECO:0000269|PubMed:12862464, ECO:0000269|PubMed:17994768, ECO:0000269|PubMed:19265437, ECO:0000269|PubMed:22538}; |
| Cofactor: | |
Name=FAD; Xref=ChEBI:CHEBI:57692; Evidence={ECO:0000269|PubMed:11867731, ECO:0000269|PubMed:16430210, ECO:0000269|PubMed:17994768, ECO:0000269|PubMed:19265437, ECO:0000269|PubMed:19926855, ECO:0000269|PubMed:22538}; |
| Activity regulation: | |
Strongly inhibited by mercury chloride and KCN. {ECO:0000269|PubMed:22538}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.54 mM for 1-nitropropane {ECO:0000269|PubMed:22538}; KM=7.4 mM for 2-nitropropane {ECO:0000269|PubMed:22538}; KM=1.0 mM for nitroethane {ECO:0000269|PubMed:22538}; KM=3.1 mM for 3-nitro-2-pentanol {ECO:0000269|PubMed:22538}; KM=0.9 mM for nitrocyclohexane {ECO:0000269|PubMed:22538}; KM=1.33 uM for FAD {ECO:0000269|PubMed:22538}; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:22538}; Temperature dependence: Optimum temperature is 40 degrees Celsius. {ECO:0000269|PubMed:22538}; |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:16430210, ECO:0000269|PubMed:17994768, ECO:0000269|PubMed:19926855, ECO:0000269|PubMed:22538}. |
| Induction: | |
Up-regulated by nitroethane. {ECO:0000269|PubMed:22538}. |
| Similarity: | |
Belongs to the acyl-CoA dehydrogenase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.