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UniProt functional annotation for P0A7E9 | ||
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| UniProt code: P0A7E9. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the reversible phosphorylation of UMP to UDP, with ATP as the most efficient phosphate donor. {ECO:0000269|PubMed:7711027}. |
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| Catalytic activity: | |
Reaction=ATP + UMP = ADP + UDP; Xref=Rhea:RHEA:24400, ChEBI:CHEBI:30616, ChEBI:CHEBI:57865, ChEBI:CHEBI:58223, ChEBI:CHEBI:456216; EC=2.7.4.22; |
| Activity regulation: | |
Allosterically activated by GTP. Competitively inhibited by magnesium-free UTP. Magnesium-bound UTP is unable to inhibit enzyme activity. {ECO:0000269|PubMed:15857829, ECO:0000269|PubMed:17210578}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=120 uM for ATP {ECO:0000269|PubMed:7711027}; KM=50 uM for UMP {ECO:0000269|PubMed:7711027}; Vmax=96 umol/min/mg enzyme {ECO:0000269|PubMed:7711027}; |
| Pathway: | |
Pyrimidine metabolism; CTP biosynthesis via de novo pathway; UDP from UMP (UMPK route): step 1/1. |
| Subunit: | |
Homohexamer. {ECO:0000269|PubMed:15857829, ECO:0000269|PubMed:7711027}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:9922246}. Note=Is predominantly localized near the bacterial membranes. |
| Miscellaneous: | |
The peripheral distribution of PyrH is related most probably to its role in the synthesis of membrane sugar components. |
| Similarity: | |
Belongs to the UMP kinase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.