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UniProt functional annotation for P0A7K2 | ||
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| UniProt code: P0A7K2. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
The binding site for several of the GTPase factors involved in protein synthesis (IF-2, EF-Tu, EF-G and RF3). Is thus essential for accurate translation. Deletion of 1 of the L12 dimers from the ribosome (by deleting the binding site on L10) leads to decreased IF-2 association with the 70S ribosome and decreased stimulation of the GTPase activity of EF-G. {ECO:0000269|PubMed:15989950, ECO:0000269|PubMed:22102582}. |
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| Subunit: | |
Homodimer. Part of the 50S ribosomal subunit; present in 4 copies per ribosome. L7/L12 forms dimers with an elongated shape. Two dimers associate with a copy of L10 to form part of the ribosomal stalk (called L8). The ribosomal stalk helps the ribosome interact with GTP- bound translation factors. Forms a pentameric L10(L12)2(L12)2 complex, where L10 forms an elongated spine to which 2 L12 dimers bind in a sequential fashion. {ECO:0000269|PubMed:15923259, ECO:0000269|PubMed:15989950}. |
| Ptm: | |
Acetylation of Ser-2 converts L12 to L7. |
| Ptm: | |
Lys-82 was found to be 50% monomethylated. {ECO:0000269|PubMed:4573678}. |
| Mass spectrometry: | |
Mass=12206.7; Method=MALDI; Note=Non-methylated.; Evidence={ECO:0000269|PubMed:10094780}; |
| Mass spectrometry: | |
Mass=66643; Mass_error=13; Method=Electrospray; Note=Isolated L10(L12)4.; Evidence={ECO:0000269|PubMed:15923259}; |
| Mass spectrometry: | |
Mass=12223; Mass_error=1; Method=Electrospray; Note=L7, acetylated Ser-2.; Evidence={ECO:0000269|PubMed:15923259}; |
| Mass spectrometry: | |
Mass=12164; Mass_error=1; Method=Electrospray; Note=L12, unacetylated.; Evidence={ECO:0000269|PubMed:15923259}; |
| Miscellaneous: | |
Ribosomal protein L8 appears to be an aggregate of ribosomal proteins L7/L12 and L10. {ECO:0000269|PubMed:773698}. |
| Similarity: | |
Belongs to the bacterial ribosomal protein bL12 family. {ECO:0000255|HAMAP-Rule:MF_00368}. |
Annotations taken from UniProtKB at the EBI.