UniProt functional annotation for Q5SM01



	UniProt functional annotation for Q5SM01

UniProt code: Q5SM01.

Organism: Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).

Taxonomy: Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.

Function: Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA (Probable). In endogenous ribosomes interacts with P-site tRNA and 23S rRNA (PubMed:18988853, PubMed:20421507). In the presence of truncated mRNA in the 70S ribosome, ArfA and RF2 interact such that the GGQ peptide hydrolysis motif of RF2 rises into the peptidyl-transferase center and releases the ribosome (By similarity). Recruited to stalled E.coli 70S ribosomes by E.coli ArfA, but cannot be functionally accomodated in the peptidyl-transferase center (PubMed:27934701, PubMed:28077875). Note T.thermophilus probably does not encode arfA (Ref.1). {ECO:0000250|UniProtKB:P07012, ECO:0000269|PubMed:18988853, ECO:0000269|PubMed:20421507, ECO:0000269|PubMed:27934701, ECO:0000269|PubMed:28077875, ECO:0000305|PubMed:16377566, ECO:0000305|Ref.1}.

Subunit: Interacts with the ribosome (PubMed:16377566, PubMed:17272297, PubMed:18988853, PubMed:20421507). Interacts with ribosomal protein L11 (PubMed:18988853). Recruited to stalled E.coli ribosomes by E.coli ArfA (PubMed:27934701, PubMed:28077875). {ECO:0000269|PubMed:16377566, ECO:0000269|PubMed:17272297, ECO:0000269|PubMed:18988853, ECO:0000269|PubMed:20421507, ECO:0000269|PubMed:28077875, ECO:0000305|PubMed:27934701}.

Subcellular location: Cytoplasm {ECO:0000305|PubMed:17272297}.

Similarity: Belongs to the prokaryotic/mitochondrial release factor family.

Annotations taken from UniProtKB at the EBI.


Organism:		Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8).
Taxonomy:		Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus.



Function:		Peptide chain release factor 2 directs the termination of translation in response to the peptide chain termination codons UGA and UAA (Probable). In endogenous ribosomes interacts with P-site tRNA and 23S rRNA (PubMed:18988853, PubMed:20421507). In the presence of truncated mRNA in the 70S ribosome, ArfA and RF2 interact such that the GGQ peptide hydrolysis motif of RF2 rises into the peptidyl-transferase center and releases the ribosome (By similarity). Recruited to stalled E.coli 70S ribosomes by E.coli ArfA, but cannot be functionally accomodated in the peptidyl-transferase center (PubMed:27934701, PubMed:28077875). Note T.thermophilus probably does not encode arfA (Ref.1). {ECO:0000250\|UniProtKB:P07012, ECO:0000269\|PubMed:18988853, ECO:0000269\|PubMed:20421507, ECO:0000269\|PubMed:27934701, ECO:0000269\|PubMed:28077875, ECO:0000305\|PubMed:16377566, ECO:0000305\|Ref.1}.


Subunit:		Interacts with the ribosome (PubMed:16377566, PubMed:17272297, PubMed:18988853, PubMed:20421507). Interacts with ribosomal protein L11 (PubMed:18988853). Recruited to stalled E.coli ribosomes by E.coli ArfA (PubMed:27934701, PubMed:28077875). {ECO:0000269\|PubMed:16377566, ECO:0000269\|PubMed:17272297, ECO:0000269\|PubMed:18988853, ECO:0000269\|PubMed:20421507, ECO:0000269\|PubMed:28077875, ECO:0000305\|PubMed:27934701}.
Subcellular location:		Cytoplasm {ECO:0000305\|PubMed:17272297}.
Similarity:		Belongs to the prokaryotic/mitochondrial release factor family.