PDBsum entry 2b9m

Ribosome

PDB id: 2b9m

Contents

Protein chains

234 a.a.

206 a.a.

208 a.a.

150 a.a.

101 a.a.

155 a.a.

138 a.a.

127 a.a.

98 a.a.

119 a.a.

124 a.a.

125 a.a.

60 a.a.

88 a.a.

83 a.a.

104 a.a.

73 a.a.

80 a.a.

99 a.a.

24 a.a.

365 a.a.*

DNA/RNA

* C-alpha coords only

References listed in PDB file

Key reference

Title

Crystal structures of the ribosome in complex with release factors rf1 and rf2 bound to a cognate stop codon.

Authors

S.Petry, D.E.Brodersen, F.V.Murphy, C.M.Dunham, M.Selmer, M.J.Tarry, A.C.Kelley, V.Ramakrishnan.

Ref.

Cell, 2005, 123, 1255-1266. [DOI no: 10.1016/j.cell.2005.09.039]

PubMed id

16377566

Abstract

During protein synthesis, translational release factors catalyze the release of the polypeptide chain when a stop codon on the mRNA reaches the A site of the ribosome. The detailed mechanism of this process is currently unknown. We present here the crystal structures of the ribosome from Thermus thermophilus with RF1 and RF2 bound to their cognate stop codons, at resolutions of 5.9 Angstrom and 6.7 Angstrom, respectively. The structures reveal details of interactions of the factors with the ribosome and mRNA, including elements previously implicated in decoding and peptide release. They also shed light on conformational changes both in the factors and in the ribosome during termination. Differences seen in the interaction of RF1 and RF2 with the L11 region of the ribosome allow us to rationalize previous biochemical data. Finally, this work demonstrates the feasibility of crystallizing ribosomes with bound factors at a defined state along the translational pathway.

Figure 4.
Figure 4. Interaction of RF1 with the Decoding Center of the 30S Subunit, with Overview on the Left and Details on the Right

Figure 6.
Figure 6. Interaction of RF1 and RF2 with the L11 Region of the Ribosome

The above figures are reprinted by permission from Cell Press: Cell (2005, 123, 1255-1266) copyright 2005.