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PDBsum entry 2b97
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Surface active protein
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PDB id
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2b97
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References listed in PDB file
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Key reference
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Title
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Hydrophobin hfbii in detail: ultrahigh-Resolution structure at 0.75 a.
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Authors
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J.Hakanpää,
M.Linder,
A.Popov,
A.Schmidt,
J.Rouvinen.
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Ref.
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Acta Crystallogr D Biol Crystallogr, 2006,
62,
356-367.
[DOI no: ]
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PubMed id
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Abstract
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Hydrophobins are small proteins secreted by filamentous fungi that have a unique
ability to spontaneously form amphiphilic layers. Hydrophobins have only
recently been structurally characterized through the first crystal structure
determination of a protein of this class, Trichoderma reesei hydrophobin HFBII
[Hakanpää, Paananen et al. (2004), J. Biol. Chem. 279, 534-539]. The
resolution of the HFBII structure has now been extended to an ultrahigh
resolution of 0.75 A. The structure was refined conventionally and multipole
refinement has been initiated. The ultrahigh-resolution structure is analyzed
here in detail and comparison is made to the previous atomic resolution
structure of the same protein as well as to other ultrahigh-resolution
structures found in the Protein Data Bank.
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Figure 1.
Figure 1
(a) Sequence of T. reesei hydrophobin HFBII, with yellow colour indicating the conserved
cysteine residues and red colour highlighting the residues of the hydrophobic patch. The
signal sequence is shown in blue. (b) The structure of T. reeesei hydrophobin HFBII;
disulfide bridges are highlighted in yellow and the hydrophobic patch in red. The figure
was produced using PyMOL (DeLano, 2002[66] [DeLano, W. L. (2002). The PyMOL Molecular
Graphics System. DeLano Scientific, CA, USA. http://www.pymol.org .]-[67][bluearr.gif] ).
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Figure 3.
Figure 3
Omit map of HFBII ultrahigh-resolution structure showing density at the hydrogen positions
of Ile22, chain B. The figure was produced using PyMOL (DeLano, 2002[137] [DeLano, W. L.
(2002). The PyMOL Molecular Graphics System. DeLano Scientific, CA, USA.
http://www.pymol.org .]-[138][bluearr.gif] ). 2F[o] - F[c] maps at 1.0 [139][sigma] are
shown in blue and F[o] - F[c] maps at 2.0 [140][sigma] in pink.
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The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(2006,
62,
356-367)
copyright 2006.
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