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UniProt functional annotation for P21673 | ||
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| UniProt code: P21673. |
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| Organism: | |
Homo sapiens (Human). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Homo. |
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| Function: | |
Enzyme which catalyzes the acetylation of polyamines (PubMed:15283699, PubMed:16455797, PubMed:17516632). Substrate specificity: norspermidine = spermidine >> spermine > N(1)- acetylspermine > putrescine (PubMed:17516632). This highly regulated enzyme allows a fine attenuation of the intracellular concentration of polyamines (PubMed:16455797). Also involved in the regulation of polyamine transport out of cells (PubMed:16455797). Acts on 1,3- diaminopropane, 1,5-diaminopentane, putrescine, spermidine (forming N(1)- and N(8)-acetylspermidine), spermine, N(1)-acetylspermidine and N(8)-acetylspermidine (PubMed:16455797, PubMed:17516632). {ECO:0000269|PubMed:15283699, ECO:0000269|PubMed:16455797, ECO:0000269|PubMed:17516632}. |
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| Catalytic activity: | |
Reaction=acetyl-CoA + an alkane-alpha,omega-diamine = an N- acetylalkane-alpha,omega-diamine + CoA + H(+); Xref=Rhea:RHEA:11116, Rhea:RHEA-COMP:9766, Rhea:RHEA-COMP:9767, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:70977, ChEBI:CHEBI:70988; EC=2.3.1.57; Evidence={ECO:0000269|PubMed:15283699, ECO:0000269|PubMed:16455797, ECO:0000269|PubMed:17516632}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:11117; Evidence={ECO:0000269|PubMed:15283699, ECO:0000269|PubMed:16455797, ECO:0000269|PubMed:17516632}; |
| Catalytic activity: | |
Reaction=acetyl-CoA + spermidine = CoA + H(+) + N(1)-acetylspermidine; Xref=Rhea:RHEA:28150, ChEBI:CHEBI:15378, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:57834, ChEBI:CHEBI:58324; EC=2.3.1.57; Evidence={ECO:0000269|PubMed:15283699, ECO:0000269|PubMed:17516632}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:28151; Evidence={ECO:0000269|PubMed:15283699, ECO:0000269|PubMed:17516632}; |
| Catalytic activity: | |
Reaction=acetyl-CoA + spermine = CoA + H(+) + N(1)-acetylspermine; Xref=Rhea:RHEA:33099, ChEBI:CHEBI:15378, ChEBI:CHEBI:45725, ChEBI:CHEBI:57287, ChEBI:CHEBI:57288, ChEBI:CHEBI:58101; EC=2.3.1.57; Evidence={ECO:0000269|PubMed:15283699, ECO:0000269|PubMed:17516632}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:33100; Evidence={ECO:0000269|PubMed:15283699, ECO:0000269|PubMed:17516632}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=3.8 uM for acetyl-coenzyme A {ECO:0000269|PubMed:17516632}; KM=5.7 uM for spermine {ECO:0000269|PubMed:17516632}; KM=3.7 uM for spermine {ECO:0000269|PubMed:15283699}; KM=22 uM for spermidine {ECO:0000269|PubMed:17516632}; KM=58 uM for spermidine {ECO:0000269|PubMed:15283699}; Note=kcat is 8.7 sec(-1) with spermidine as substrate (PubMed:15283699). kcat is 5 sec(-1) with spermine as substrate (PubMed:15283699). {ECO:0000269|PubMed:15283699}; pH dependence: Optimum pH is 8.0. {ECO:0000269|PubMed:17516632}; |
| Pathway: | |
Amine and polyamine degradation; putrescine degradation; N- acetylputrescine from putrescine: step 1/1. {ECO:0000269|PubMed:16455797, ECO:0000269|PubMed:17516632}. |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:16455797, ECO:0000269|PubMed:16544326, ECO:0000269|PubMed:17516632}. |
| Subcellular location: | |
Cytoplasm, cytosol {ECO:0000305|PubMed:1985966, ECO:0000305|PubMed:2241897}. |
| Disease: | |
Note=Overexpression of SAT1 and the consequent putrescine accumulation might play a role in the pathogenesis of keratosis follicularis spinulosa decalvans. {ECO:0000269|PubMed:12215835}. |
| Similarity: | |
Belongs to the acetyltransferase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.