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PDBsum entry 2aph
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Immune system
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PDB id
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2aph
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References listed in PDB file
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Key reference
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Title
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Crystal structure of human peptidoglycan recognition protein i alpha bound to a muramyl pentapeptide from gram-Positive bacteria.
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Authors
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R.Guan,
P.H.Brown,
C.P.Swaminathan,
A.Roychowdhury,
G.J.Boons,
R.A.Mariuzza.
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Ref.
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Protein Sci, 2006,
15,
1199-1206.
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PubMed id
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Abstract
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Peptidoglycan recognition proteins (PGRPs) are pattern recognition receptors of
the innate immune system that bind bacterial peptidoglycans (PGNs). We
determined the crystal structure, to 2.1 A resolution, of the C-terminal
PGN-binding domain of human PGRP-I alpha in complex with a muramyl pentapeptide
(MPP) from Gram-positive bacteria containing a complete peptide stem
(L-Ala-D-isoGln-L-Lys-D-Ala-D-Ala). The structure reveals important features not
observed previously in the complex between PGRP-I alpha and a muramyl tripeptide
lacking D-Ala at stem positions 4 and 5. Most notable are ligand-induced
structural rearrangements in the PGN-binding site that are essential for entry
of the C-terminal portion of the peptide stem and for locking MPP in the binding
groove. We propose that similar structural rearrangements to accommodate the PGN
stem likely characterize many PGRPs, both mammalian and insect.
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