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UniProt functional annotation for Q873X9 | ||
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| UniProt code: Q873X9. |
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| Organism: | |
Neosartorya fumigata (Aspergillus fumigatus). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Pezizomycotina; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus subgen. Fumigati. |
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| Function: | |
Major secreted chitinase involved in the degradation of chitin, a component of the cell walls of fungi and exoskeletal elements of some animals (including worms and arthropods). Plays a role in the morphogenesis and autolysis (By similarity). {ECO:0000250}. |
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| Catalytic activity: | |
Reaction=Random endo-hydrolysis of N-acetyl-beta-D-glucosaminide (1->4)-beta-linkages in chitin and chitodextrins.; EC=3.2.1.14; Evidence={ECO:0000269|PubMed:14523125, ECO:0000269|PubMed:15664516, ECO:0000269|PubMed:16096835, ECO:0000269|PubMed:16183021, ECO:0000269|PubMed:18975073}; |
| Activity regulation: | |
Methylxanthine drugs surch as theophylline, caffeine and pentoxifylline, as well as the two cyclic peptide natural products argifin and argadin, act as specific inhibitors. {ECO:0000269|PubMed:15664516, ECO:0000269|PubMed:16183021, ECO:0000269|PubMed:16844689, ECO:0000269|PubMed:18355729}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=19.9 uM for 4-methylumbelliferyl beta-D-N,N'-diacetylchitobiose {ECO:0000269|PubMed:15664516, ECO:0000269|PubMed:18975073}; KM=2.94 mg/ml for chitosan {ECO:0000269|PubMed:15664516, ECO:0000269|PubMed:18975073}; pH dependence: Optimum pH is 5.0-7.0. {ECO:0000269|PubMed:15664516, ECO:0000269|PubMed:18975073}; Temperature dependence: Optimum temperature is 60 degrees Celsius. {ECO:0000269|PubMed:15664516, ECO:0000269|PubMed:18975073}; |
| Subcellular location: | |
Secreted {ECO:0000269|PubMed:14523125, ECO:0000269|PubMed:16096835}. |
| Induction: | |
Induced during batch culture. {ECO:0000269|PubMed:16096835}. |
| Disruption phenotype: | |
Decreases the level of chitinase activity during the autolytic phase of batch cultures. {ECO:0000269|PubMed:14523125}. |
| Similarity: | |
Belongs to the glycosyl hydrolase 18 family. Chitinase class V subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.