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UniProt functional annotation for Q05957 | ||
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| UniProt code: Q05957. |
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| Organism: | |
Dianthus caryophyllus (Carnation) (Clove pink). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; Caryophyllales; Caryophyllaceae; Caryophylleae; Dianthus. |
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| Function: | |
Catalyzes cleavage of the C(2)-C(3) bond in oxaloacetate and in (2R)-alkyl malate derivatives to form oxalate and acetate, and alkyl carboxylates and R-ketocarboxylates, respectively. {ECO:0000269|PubMed:16342929}. |
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| Catalytic activity: | |
Reaction=H2O + oxaloacetate = acetate + H(+) + oxalate; Xref=Rhea:RHEA:24432, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16452, ChEBI:CHEBI:30089, ChEBI:CHEBI:30623; EC=3.7.1.1; Evidence={ECO:0000269|PubMed:16342929}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:16342929}; Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:16342929}; Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000269|PubMed:16342929}; Name=Co(2+); Xref=ChEBI:CHEBI:48828; Evidence={ECO:0000269|PubMed:16342929}; Note=Binds 1 Mg(2+) ion per subunit. Can bind other divalent cations such as Mn(2+), Fe(2+) and Co(2+). {ECO:0000269|PubMed:16342929}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=290 uM for (R)-citramalate (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=130 uM for oxaloacetate (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=42 uM for (2R,3S)-isocitrate (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=104 uM for (2R,3R:2S,3S)-2-methyl isocitrate (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=69 uM for (2R,3S:2S,3R)-2-methyl isocitrate (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=1100 uM for (2R)-2-ethyl malate (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=26 uM for (2R,3S)-2,3-dimethyl malate (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=450 uM for (2R)-ethyl-(3S)-methyl malate (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=98 uM for (2R)-propyl-(3S)-methyl malate (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=8000 uM for (2R)-isobutyl-(3S)-methyl malate (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=19 uM for magnesium ion (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=0.7 uM for manganese ion (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=2 uM for cobalt ion (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; KM=6 uM for iron ion (at pH 7.5 and 25 degrees Celsius) {ECO:0000269|PubMed:16342929}; pH dependence: Optimum pH is 7.0. {ECO:0000269|PubMed:16342929}; |
| Subunit: | |
Homodimer and homotetramer formed by a dimer of homodimer. {ECO:0000269|PubMed:16342930}. |
| Tissue specificity: | |
Accumulates in senescing flower petals. {ECO:0000269|PubMed:8393719}. |
| Induction: | |
By ethylene. {ECO:0000269|PubMed:8393719}. |
| Similarity: | |
Belongs to the isocitrate lyase/PEP mutase superfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.