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UniProt functional annotation for P13280 | ||
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| UniProt code: P13280. |
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| Organism: | |
Oryctolagus cuniculus (Rabbit). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Lagomorpha; Leporidae; Oryctolagus. |
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| Function: | |
Self-glucosylates, via an inter-subunit mechanism, to form an oligosaccharide primer that serves as substrate for glycogen synthase. {ECO:0000269|PubMed:10049511, ECO:0000269|PubMed:1281472, ECO:0000269|PubMed:15849187, ECO:0000269|PubMed:22128147, ECO:0000269|PubMed:22226635, ECO:0000269|PubMed:2526735, ECO:0000269|PubMed:7771798, ECO:0000269|PubMed:8143846}. |
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| Catalytic activity: | |
Reaction=L-tyrosyl-[glycogenin] + UDP-alpha-D-glucose = alpha-D- glucosyl-L-tyrosyl-[glycogenin] + H(+) + UDP; Xref=Rhea:RHEA:23360, Rhea:RHEA-COMP:14604, Rhea:RHEA-COMP:14605, ChEBI:CHEBI:15378, ChEBI:CHEBI:46858, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:140573; EC=2.4.1.186; Evidence={ECO:0000269|PubMed:10049511, ECO:0000269|PubMed:1281472, ECO:0000269|PubMed:15849187, ECO:0000269|PubMed:22128147, ECO:0000269|PubMed:22226635, ECO:0000269|PubMed:2526735, ECO:0000269|PubMed:7771798, ECO:0000269|PubMed:8143846}; |
| Catalytic activity: | |
Reaction=[1,4-alpha-D-glucosyl](n)-L-tyrosyl-[glycogenin] + UDP-alpha- D-glucose = [1,4-alpha-D-glucosyl](n+1)-L-tyrosyl-[glycogenin] + H(+) + UDP; Xref=Rhea:RHEA:56560, Rhea:RHEA-COMP:14606, Rhea:RHEA- COMP:14607, ChEBI:CHEBI:15378, ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:140574; EC=2.4.1.186; Evidence={ECO:0000269|PubMed:10049511, ECO:0000269|PubMed:1281472, ECO:0000269|PubMed:15849187, ECO:0000269|PubMed:22128147, ECO:0000269|PubMed:22226635, ECO:0000269|PubMed:2526735, ECO:0000269|PubMed:7771798, ECO:0000269|PubMed:8143846}; |
| Cofactor: | |
Name=Mn(2+); Xref=ChEBI:CHEBI:29035; Evidence={ECO:0000269|PubMed:12051921, ECO:0000269|PubMed:1281472, ECO:0000269|PubMed:15849187, ECO:0000269|PubMed:22128147, ECO:0000269|PubMed:22226635, ECO:0000269|PubMed:2526735, ECO:0000269|PubMed:8143846}; Note=Divalent metal ions. Required for self-glucosylation. Manganese is the most effective. {ECO:0000269|PubMed:12051921}; |
| Pathway: | |
Glycan biosynthesis; glycogen biosynthesis. {ECO:0000269|PubMed:1281472, ECO:0000269|PubMed:15849187, ECO:0000269|PubMed:2526735}. |
| Subunit: | |
Homodimer (PubMed:12051921, PubMed:15849187, PubMed:22226635, PubMed:22128147). Interacts (via C-terminus) with glycogen synthase GYS1 (By similarity). Interacts (via C-terminus) with glycogen synthase GYS2 (By similarity). This interaction is required for GYS2-mediated glycogen synthesis (By similarity). {ECO:0000250|UniProtKB:P46976, ECO:0000250|UniProtKB:Q9R062, ECO:0000269|PubMed:12051921, ECO:0000269|PubMed:15849187, ECO:0000269|PubMed:22128147, ECO:0000269|PubMed:22226635}. |
| Tissue specificity: | |
Detected in heart, skeletal muscle, brain and testis, and at lower levels in kidney. {ECO:0000269|PubMed:1281472}. |
| Ptm: | |
Self-glycosylated by the transfer of glucose residues from UDP- glucose to itself, forming an alpha-1,4-glycan of around 10 residues attached to Tyr-195. {ECO:0000269|PubMed:10049511, ECO:0000269|PubMed:22128147, ECO:0000269|PubMed:22226635, ECO:0000269|PubMed:7771798, ECO:0000269|PubMed:8143846}. |
| Ptm: | |
Phosphorylated. {ECO:0000269|PubMed:3151442}. |
| Similarity: | |
Belongs to the glycosyltransferase 8 family. Glycogenin subfamily. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.