UniProt functional annotation for P45947



	UniProt functional annotation for P45947

UniProt code: P45947.

Organism: Bacillus subtilis (strain 168).

Taxonomy: Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.

Function: Catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)] (PubMed:16797027). In vitro, can dephosphorylate para- nitrophenyl phosphate (pNPP) (PubMed:11698660). {ECO:0000269|PubMed:11698660, ECO:0000269|PubMed:16797027}.

Catalytic activity: Reaction=[thioredoxin]-dithiol + arsenate + H(+) = [thioredoxin]- disulfide + arsenite + H2O; Xref=Rhea:RHEA:43848, Rhea:RHEA- COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29242, ChEBI:CHEBI:29950, ChEBI:CHEBI:48597, ChEBI:CHEBI:50058; EC=1.20.4.4; Evidence={ECO:0000255|HAMAP-Rule:MF_01624, ECO:0000269|PubMed:16797027};

Activity regulation: Activity is potassium and sulfate-independent. {ECO:0000269|PubMed:16797027}.

Biophysicochemical properties: Kinetic parameters: KM=54 uM for arsenate (in the presence of KCl) {ECO:0000269|PubMed:16797027}; KM=58 uM for arsenate (in the presence of NaCl) {ECO:0000269|PubMed:16797027}; KM=64 uM for arsenate (in the presence of Na(2)SO(4)) {ECO:0000269|PubMed:16797027}; KM=47 uM for arsenate (in the presence of K(2)SO(4)) {ECO:0000269|PubMed:16797027}; Note=kcat is 96 min(-1) in the presence of KCl (PubMed:16797027). kcat is 80 min(-1) in the presence of NaCl (PubMed:16797027). kcat is 120 min(-1) in the presence of Na(2)SO(4) (PubMed:16797027). kcat is 95 min(-1) in the presence of K(2)SO(4) (PubMed:16797027). {ECO:0000269|PubMed:16797027};

Subunit: Monomer. {ECO:0000269|PubMed:11698660}.

Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01624}.

Induction: Induced by arsenate, arsenite, and antimonite. {ECO:0000269|PubMed:9537360}.

Disruption phenotype: Mutant is sensitive to arsenate but is still resistant to arsenite. {ECO:0000269|PubMed:9537360}.

Similarity: Belongs to the low molecular weight phosphotyrosine protein phosphatase family. Thioredoxin-coupled ArsC subfamily. {ECO:0000255|HAMAP-Rule:MF_01624, ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Bacillus subtilis (strain 168).
Taxonomy:		Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.



Function:		Catalyzes the reduction of arsenate [As(V)] to arsenite [As(III)] (PubMed:16797027). In vitro, can dephosphorylate para- nitrophenyl phosphate (pNPP) (PubMed:11698660). {ECO:0000269\|PubMed:11698660, ECO:0000269\|PubMed:16797027}.


Catalytic activity:		Reaction=[thioredoxin]-dithiol + arsenate + H(+) = [thioredoxin]- disulfide + arsenite + H2O; Xref=Rhea:RHEA:43848, Rhea:RHEA- COMP:10698, Rhea:RHEA-COMP:10700, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:29242, ChEBI:CHEBI:29950, ChEBI:CHEBI:48597, ChEBI:CHEBI:50058; EC=1.20.4.4; Evidence={ECO:0000255\|HAMAP-Rule:MF_01624, ECO:0000269\|PubMed:16797027};
Activity regulation:		Activity is potassium and sulfate-independent. {ECO:0000269\|PubMed:16797027}.
Biophysicochemical properties:		Kinetic parameters: KM=54 uM for arsenate (in the presence of KCl) {ECO:0000269\|PubMed:16797027}; KM=58 uM for arsenate (in the presence of NaCl) {ECO:0000269\|PubMed:16797027}; KM=64 uM for arsenate (in the presence of Na(2)SO(4)) {ECO:0000269\|PubMed:16797027}; KM=47 uM for arsenate (in the presence of K(2)SO(4)) {ECO:0000269\|PubMed:16797027}; Note=kcat is 96 min(-1) in the presence of KCl (PubMed:16797027). kcat is 80 min(-1) in the presence of NaCl (PubMed:16797027). kcat is 120 min(-1) in the presence of Na(2)SO(4) (PubMed:16797027). kcat is 95 min(-1) in the presence of K(2)SO(4) (PubMed:16797027). {ECO:0000269\|PubMed:16797027};
Subunit:		Monomer. {ECO:0000269\|PubMed:11698660}.
Subcellular location:		Cytoplasm {ECO:0000255\|HAMAP-Rule:MF_01624}.
Induction:		Induced by arsenate, arsenite, and antimonite. {ECO:0000269\|PubMed:9537360}.
Disruption phenotype:		Mutant is sensitive to arsenate but is still resistant to arsenite. {ECO:0000269\|PubMed:9537360}.
Similarity:		Belongs to the low molecular weight phosphotyrosine protein phosphatase family. Thioredoxin-coupled ArsC subfamily. {ECO:0000255\|HAMAP-Rule:MF_01624, ECO:0000305}.