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UniProt functional annotation for P11544 | ||
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| UniProt code: P11544. |
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| Organism: | |
Rhodosporidium toruloides (Yeast) (Rhodotorula gracilis). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Basidiomycota; Pucciniomycotina; Microbotryomycetes; Sporidiobolales; Sporidiobolaceae; Rhodotorula. |
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| Function: | |
Catalyzes the non-oxidative deamination of L-phenylalanine and L-tyrosine to form trans-cinnamic acid and p-coumaric acid respectively with similar efficiencies. Facilitates the commitment step in phenylpropanoid pathways that produce lignins, coumarins and flavonoids. {ECO:0000269|PubMed:5102931}. |
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| Catalytic activity: | |
Reaction=L-phenylalanine = (E)-cinnamate + NH4(+); Xref=Rhea:RHEA:21384, ChEBI:CHEBI:15669, ChEBI:CHEBI:28938, ChEBI:CHEBI:58095; EC=4.3.1.25; Evidence={ECO:0000269|PubMed:5102931}; |
| Catalytic activity: | |
Reaction=L-tyrosine = (E)-4-coumarate + NH4(+); Xref=Rhea:RHEA:24906, ChEBI:CHEBI:12876, ChEBI:CHEBI:28938, ChEBI:CHEBI:58315; EC=4.3.1.25; Evidence={ECO:0000269|PubMed:5102931}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.29 mM for L-phenylalanine {ECO:0000269|PubMed:5102931, ECO:0000269|Ref.5}; KM=0.18 mM for L-tyrosine {ECO:0000269|PubMed:5102931, ECO:0000269|Ref.5}; pH dependence: Optimum pH is 8.5. {ECO:0000269|PubMed:5102931, ECO:0000269|Ref.5}; |
| Pathway: | |
Phenylpropanoid metabolism; trans-cinnamate biosynthesis; trans-cinnamate from L-phenylalanine: step 1/1. |
| Subunit: | |
Homotetramer. Dimer of dimers. {ECO:0000269|PubMed:15350127, ECO:0000269|Ref.5}. |
| Subcellular location: | |
Cytoplasm {ECO:0000305}. |
| Ptm: | |
Contains an active site 4-methylidene-imidazol-5-one (MIO), which is formed autocatalytically by cyclization and dehydration of residues Ala-Ser-Gly. |
| Similarity: | |
Belongs to the PAL/histidase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.