UniProt functional annotation for P0AFY8



	UniProt functional annotation for P0AFY8

UniProt code: P0AFY8.

Organism: Escherichia coli (strain K12).

Taxonomy: Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.

Function: Negative regulator of replication initiation, which contributes to regulation of DNA replication and ensures that replication initiation occurs exactly once per chromosome per cell cycle. Binds to pairs of hemimethylated GATC sequences in the oriC region, thus preventing assembly of replication proteins and re- initiation at newly replicated origins. Repression is relieved when the region becomes fully methylated. Can also bind to hemimethylated GATC sequences outside of oriC region. Binds, with less affinity, to fully methylated GATC sites and affects timing of replication. May play a role in chromosome organization and gene regulation. {ECO:0000255|HAMAP-Rule:MF_00908, ECO:0000269|PubMed:10931282, ECO:0000269|PubMed:11080170, ECO:0000269|PubMed:20689753, ECO:0000269|PubMed:7553853, ECO:0000269|PubMed:7891562, ECO:0000269|PubMed:8011018}.

Subunit: Homodimer. Polymerizes to form helical filaments. Dimerization is sufficient for DNA-binding, but oligomerization is essential for function. {ECO:0000255|HAMAP-Rule:MF_00908, ECO:0000269|PubMed:12379844, ECO:0000269|PubMed:14704346, ECO:0000269|PubMed:15933720, ECO:0000269|PubMed:19304745}.

Subcellular location: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00908, ECO:0000269|PubMed:9660922}. Note=Localization is cell cycle-dependent. Localizes at midcell in newborn cells, then migrates in opposite directions toward cell quarter sites and remains tethered there until the cell divides.

Domain: The N-terminal domain is required for multimerization. The C- terminal domain is involved in DNA-binding. {ECO:0000269|PubMed:12379844, ECO:0000269|PubMed:12507506, ECO:0000269|PubMed:19304745}.

Similarity: Belongs to the SeqA family. {ECO:0000255|HAMAP- Rule:MF_00908}.

Annotations taken from UniProtKB at the EBI.


Organism:		Escherichia coli (strain K12).
Taxonomy:		Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia.



Function:		Negative regulator of replication initiation, which contributes to regulation of DNA replication and ensures that replication initiation occurs exactly once per chromosome per cell cycle. Binds to pairs of hemimethylated GATC sequences in the oriC region, thus preventing assembly of replication proteins and re- initiation at newly replicated origins. Repression is relieved when the region becomes fully methylated. Can also bind to hemimethylated GATC sequences outside of oriC region. Binds, with less affinity, to fully methylated GATC sites and affects timing of replication. May play a role in chromosome organization and gene regulation. {ECO:0000255\|HAMAP-Rule:MF_00908, ECO:0000269\|PubMed:10931282, ECO:0000269\|PubMed:11080170, ECO:0000269\|PubMed:20689753, ECO:0000269\|PubMed:7553853, ECO:0000269\|PubMed:7891562, ECO:0000269\|PubMed:8011018}.


Subunit:		Homodimer. Polymerizes to form helical filaments. Dimerization is sufficient for DNA-binding, but oligomerization is essential for function. {ECO:0000255\|HAMAP-Rule:MF_00908, ECO:0000269\|PubMed:12379844, ECO:0000269\|PubMed:14704346, ECO:0000269\|PubMed:15933720, ECO:0000269\|PubMed:19304745}.
Subcellular location:		Cytoplasm {ECO:0000255\|HAMAP-Rule:MF_00908, ECO:0000269\|PubMed:9660922}. Note=Localization is cell cycle-dependent. Localizes at midcell in newborn cells, then migrates in opposite directions toward cell quarter sites and remains tethered there until the cell divides.
Domain:		The N-terminal domain is required for multimerization. The C- terminal domain is involved in DNA-binding. {ECO:0000269\|PubMed:12379844, ECO:0000269\|PubMed:12507506, ECO:0000269\|PubMed:19304745}.
Similarity:		Belongs to the SeqA family. {ECO:0000255\|HAMAP- Rule:MF_00908}.