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UniProt functional annotation for P77541 | ||
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| UniProt code: P77541. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Involved in the catabolism of short chain fatty acids (SCFA) via the 2-methylcitrate cycle I (propionate degradation route). Catalyzes the thermodynamically favored C-C bond cleavage of (2R,3S)-2- methylisocitrate to yield pyruvate and succinate via an alpha-carboxy- carbanion intermediate. {ECO:0000255|HAMAP-Rule:MF_01939, ECO:0000269|PubMed:11422389, ECO:0000269|PubMed:15723538}. |
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| Catalytic activity: | |
Reaction=(2S,3R)-3-hydroxybutane-1,2,3-tricarboxylate = pyruvate + succinate; Xref=Rhea:RHEA:16809, ChEBI:CHEBI:15361, ChEBI:CHEBI:30031, ChEBI:CHEBI:57429; EC=4.1.3.30; Evidence={ECO:0000255|HAMAP-Rule:MF_01939, ECO:0000269|PubMed:11422389, ECO:0000269|PubMed:15723538}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000255|HAMAP-Rule:MF_01939, ECO:0000269|PubMed:11422389, ECO:0000269|PubMed:12706720, ECO:0000269|PubMed:15723538}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=35 uM for magnesium {ECO:0000269|PubMed:11422389, ECO:0000269|PubMed:15723538}; KM=19 uM for threo-2-methylisocitrate (a mixture of (2R,3S)- and (2S,3R)-2-methylisocitrate in presence of 2 mM magnesium at pH 7 and 30 degrees Celsius) {ECO:0000269|PubMed:11422389, ECO:0000269|PubMed:15723538}; Note=Kcat is 12 sec(-1) for methylisocitrate lyase with threo-2- methylisocitrate as substrate (mixture of (2R,3S)- and (2S,3R)-2- methylisocitrate at pH 7 and 30 degrees Celsius). {ECO:0000269|PubMed:15723538}; |
| Pathway: | |
Organic acid metabolism; propanoate degradation. {ECO:0000255|HAMAP-Rule:MF_01939, ECO:0000305|PubMed:9325432}. |
| Subunit: | |
Homotetramer; dimer of dimers. {ECO:0000255|HAMAP- Rule:MF_01939, ECO:0000269|PubMed:12706720, ECO:0000269|PubMed:15723538}. |
| Similarity: | |
Belongs to the isocitrate lyase/PEP mutase superfamily. Methylisocitrate lyase family. {ECO:0000255|HAMAP-Rule:MF_01939, ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.