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UniProt functional annotation for Q8ZWV0 | ||
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| UniProt code: Q8ZWV0. |
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| Organism: | |
Pyrobaculum aerophilum (strain ATCC 51768 / IM2 / DSM 7523 / JCM 9630 / NBRC 100827). |
| Taxonomy: | |
Archaea; Crenarchaeota; Thermoprotei; Thermoproteales; Thermoproteaceae; Pyrobaculum. |
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| Function: | |
Catalyzes the isomerization of both glucose 6-phosphate and epimeric mannose 6-phosphate at a similar catalytic efficiency. |
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| Catalytic activity: | |
Reaction=aldehydo-D-glucose 6-phosphate = keto-D-fructose 6-phosphate; Xref=Rhea:RHEA:11816, ChEBI:CHEBI:57579, ChEBI:CHEBI:57584; EC=5.3.1.9; |
| Catalytic activity: | |
Reaction=D-mannose 6-phosphate = D-fructose 6-phosphate; Xref=Rhea:RHEA:12356, ChEBI:CHEBI:58735, ChEBI:CHEBI:61527; EC=5.3.1.8; |
| Activity regulation: | |
Inhibited by 5-phosphoarabinonate (PAB) and 6- phosphogluconate. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=2.7 mM for glucose 6-phosphate (at 50 degrees Celsius) {ECO:0000269|PubMed:15290326}; KM=0.3 mM for fructose 6-phosphate (at 50 degrees Celsius) {ECO:0000269|PubMed:15290326}; KM=1.9 mM for glucose 6-phosphate (at 80 degrees Celsius) {ECO:0000269|PubMed:15290326}; KM=0.06 mM for fructose 6-phosphate (at 80 degrees Celsius) {ECO:0000269|PubMed:15290326}; KM=0.49 mM for mannose 6-phosphate (at 80 degrees Celsius) {ECO:0000269|PubMed:15290326}; Vmax=50 mmol/min/mg enzyme with glucose 6-phosphate as substrate (at 50 degrees Celsius) {ECO:0000269|PubMed:15290326}; Vmax=31.3 mmol/min/mg enzyme with fructose 6-phosphate as substrate (at 50 degrees Celsius) {ECO:0000269|PubMed:15290326}; Vmax=150 mmol/min/mg enzyme with glucose 6-phosphate as substrate (at 80 degrees Celsius) {ECO:0000269|PubMed:15290326}; Vmax=109 mmol/min/mg enzyme with fructose 6-phosphate as substrate (at 80 degrees Celsius) {ECO:0000269|PubMed:15290326}; Vmax=117 mmol/min/mg enzyme with mannose 6-phosphate as substrate (at 80 degrees Celsius) {ECO:0000269|PubMed:15290326}; pH dependence: Optimum pH is 7.4. {ECO:0000269|PubMed:15290326}; Temperature dependence: Optimum temperature is 100 degrees Celsius. {ECO:0000269|PubMed:15290326}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:15252053}. |
| Miscellaneous: | |
The lack of any movement in response to the binding of ligand may be due to its inherent thermostability, which would tend to restrict any flexibility in the protein. |
| Similarity: | |
Belongs to the PGI/PMI family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.