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PDBsum entry 1wrb
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the n-Terminal reca-Like domain of a dead-Box RNA helicase, The dugesia japonica vasa-Like gene b protein.
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Authors
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K.Kurimoto,
Y.Muto,
N.Obayashi,
T.Terada,
M.Shirouzu,
T.Yabuki,
M.Aoki,
E.Seki,
T.Matsuda,
T.Kigawa,
H.Okumura,
A.Tanaka,
N.Shibata,
M.Kashikawa,
K.Agata,
S.Yokoyama.
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Ref.
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J Struct Biol, 2005,
150,
58-68.
[DOI no: ]
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PubMed id
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Abstract
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The Dugesia japonica vasa-like gene B (DjVLGB) protein is a DEAD-box RNA
helicase of a planarian, which is well known for its strong regenerative
capacity. DjVLGB shares sequence similarity to the Drosophila germ-line-specific
DEAD-box RNA helicase Vasa, and even higher similarity to its paralogue, mouse
PL10. In this study, we solved the crystal structure of the DjVLGB N-terminal
RecA-like domain. The overall fold and the structures of the putative ATPase
active site of the DjVLGB N-terminal RecA-like domain are similar to those of
the previously reported DEAD-box RNA helicase structures. In contrast, the
surface structure of the side opposite to the putative ATPase active site is
different from those of the other DEAD-box RNA helicases; the characteristic
hydrophobic pockets are formed with aromatic and proline residues. These
pocket-forming residues are conserved in the PL10-subfamily proteins, but less
conserved in the Vasa orthologues and not conserved in the DEAD-box RNA
helicases. Therefore, the structural features that we found are characteristic
of the PL10-subfamily proteins and might contribute to their biological roles in
germ-line development.
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