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PDBsum entry 1wid
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DNA binding protein
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PDB id
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1wid
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References listed in PDB file
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Key reference
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Title
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Solution structure of the b3 DNA binding domain of the arabidopsis cold-Responsive transcription factor rav1.
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Authors
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K.Yamasaki,
T.Kigawa,
M.Inoue,
M.Tateno,
T.Yamasaki,
T.Yabuki,
M.Aoki,
E.Seki,
T.Matsuda,
Y.Tomo,
N.Hayami,
T.Terada,
M.Shirouzu,
T.Osanai,
A.Tanaka,
M.Seki,
K.Shinozaki,
S.Yokoyama.
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Ref.
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Plant Cell, 2004,
16,
3448-3459.
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PubMed id
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Abstract
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The B3 DNA binding domain is shared amongst various plant-specific transcription
factors, including factors involved in auxin-regulated and abscisic
acid-regulated transcription. Herein, we report the NMR solution structure of
the B3 domain of the Arabidopsis thaliana cold-responsive transcription factor
RAV1. The structure consists of a seven-stranded open beta-barrel and two
alpha-helices located at the ends of the barrel and is significantly similar to
the structure of the noncatalytic DNA binding domain of the restriction enzyme
EcoRII. An NMR titration experiment revealed a DNA recognition interface that
enabled us to propose a structural model of the protein-DNA complex. The
locations of the DNA-contacting residues are also likely to be similar to those
of the EcoRII DNA binding domain.
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