UniProt functional annotation for O54288



	UniProt functional annotation for O54288

UniProt code: O54288.

Organism: Saccharolobus solfataricus (Sulfolobus solfataricus).

Taxonomy: Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Saccharolobus.

Function: Involved in the degradation of glucose and galactose via the Entner-Doudoroff pathway. Catalyzes the reversible cleavage of 2-keto- 3-deoxy-6-phosphogluconate (KDPG) and 2-keto-3-deoxygluconate (KDG) forming pyruvate and glyceraldehyde 3-phosphate or glyceraldehyde, respectively. It is also able to catalyze the reversible cleavage of 2- keto-3-deoxy-6-phosphogalactonate (KDPGal) and 2-keto-3- deoxygalactonate (KDGal). It is equally active with both D- and L- glyceraldehyde. {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}.

Catalytic activity: Reaction=2-dehydro-3-deoxy-6-phospho-D-gluconate = D-glyceraldehyde 3- phosphate + pyruvate; Xref=Rhea:RHEA:17089, ChEBI:CHEBI:15361, ChEBI:CHEBI:57569, ChEBI:CHEBI:59776; EC=4.1.2.55; Evidence={ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170};

Catalytic activity: Reaction=2-dehydro-3-deoxy-6-phospho-D-galactonate = D-glyceraldehyde 3-phosphate + pyruvate; Xref=Rhea:RHEA:24464, ChEBI:CHEBI:15361, ChEBI:CHEBI:58298, ChEBI:CHEBI:59776; EC=4.1.2.55; Evidence={ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170};

Biophysicochemical properties: Kinetic parameters: KM=0.1 mM for KDPG (at 60 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; KM=0.17 mM for KDPGal (at 60 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; KM=3.9 mM for pyruvate (at 70 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; KM=3.9 mM for D-glyceraldehyde (at 70 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; KM=5.2 mM for D,L-glyceraldehyde (at 70 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; KM=7.1 mM for L-glyceraldehyde (at 70 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; KM=9.9 mM for KDGal (at 60 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; KM=25.7 mM for KDG (at 60 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; Vmax=12.3 umol/min/mg enzyme with KDGal as substrate (at 60 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; Vmax=15.7 umol/min/mg enzyme with pyruvate as substrate (at 70 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; Vmax=17.1 umol/min/mg enzyme with D,L-glyceraldehyde as substrate (at 70 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; Vmax=18 umol/min/mg enzyme with D-glyceraldehyde as substrate (at 70 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; Vmax=18 umol/min/mg enzyme with L-glyceraldehyde as substrate (at 70 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; Vmax=51.4 umol/min/mg enzyme with KDG as substrate (at 60 degrees Celsius and at pH 6) {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030}; Note=Kcat is 28.2 (sec-1) for KDG and 6.8 (sec-1) for KDGal.; Temperature dependence: Extremely thermostable. {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:12824170, ECO:0000269|PubMed:16330030};

Pathway: Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro- 3-deoxy-D-gluconate: step 2/2.

Subunit: Homotetramer; dimer of dimers. {ECO:0000269|PubMed:10527934, ECO:0000269|PubMed:15265860, ECO:0000269|Ref.5}.

Miscellaneous: The metabolic pathway in Sulfolobus species contrasts with the situation observed in other microorganisms where separate enzymes exist for the catabolism of the two sugars, glucose and galactose. {ECO:0000305|PubMed:12824170}.

Similarity: Belongs to the DapA family. KDPG aldolase subfamily. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Saccharolobus solfataricus (Sulfolobus solfataricus).
Taxonomy:		Archaea; Crenarchaeota; Thermoprotei; Sulfolobales; Sulfolobaceae; Saccharolobus.



Function:		Involved in the degradation of glucose and galactose via the Entner-Doudoroff pathway. Catalyzes the reversible cleavage of 2-keto- 3-deoxy-6-phosphogluconate (KDPG) and 2-keto-3-deoxygluconate (KDG) forming pyruvate and glyceraldehyde 3-phosphate or glyceraldehyde, respectively. It is also able to catalyze the reversible cleavage of 2- keto-3-deoxy-6-phosphogalactonate (KDPGal) and 2-keto-3- deoxygalactonate (KDGal). It is equally active with both D- and L- glyceraldehyde. {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}.


Catalytic activity:		Reaction=2-dehydro-3-deoxy-6-phospho-D-gluconate = D-glyceraldehyde 3- phosphate + pyruvate; Xref=Rhea:RHEA:17089, ChEBI:CHEBI:15361, ChEBI:CHEBI:57569, ChEBI:CHEBI:59776; EC=4.1.2.55; Evidence={ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170};
Catalytic activity:		Reaction=2-dehydro-3-deoxy-6-phospho-D-galactonate = D-glyceraldehyde 3-phosphate + pyruvate; Xref=Rhea:RHEA:24464, ChEBI:CHEBI:15361, ChEBI:CHEBI:58298, ChEBI:CHEBI:59776; EC=4.1.2.55; Evidence={ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170};
Biophysicochemical properties:		Kinetic parameters: KM=0.1 mM for KDPG (at 60 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; KM=0.17 mM for KDPGal (at 60 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; KM=3.9 mM for pyruvate (at 70 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; KM=3.9 mM for D-glyceraldehyde (at 70 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; KM=5.2 mM for D,L-glyceraldehyde (at 70 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; KM=7.1 mM for L-glyceraldehyde (at 70 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; KM=9.9 mM for KDGal (at 60 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; KM=25.7 mM for KDG (at 60 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; Vmax=12.3 umol/min/mg enzyme with KDGal as substrate (at 60 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; Vmax=15.7 umol/min/mg enzyme with pyruvate as substrate (at 70 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; Vmax=17.1 umol/min/mg enzyme with D,L-glyceraldehyde as substrate (at 70 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; Vmax=18 umol/min/mg enzyme with D-glyceraldehyde as substrate (at 70 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; Vmax=18 umol/min/mg enzyme with L-glyceraldehyde as substrate (at 70 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; Vmax=51.4 umol/min/mg enzyme with KDG as substrate (at 60 degrees Celsius and at pH 6) {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030}; Note=Kcat is 28.2 (sec-1) for KDG and 6.8 (sec-1) for KDGal.; Temperature dependence: Extremely thermostable. {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:12824170, ECO:0000269\|PubMed:16330030};
Pathway:		Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro- 3-deoxy-D-gluconate: step 2/2.
Subunit:		Homotetramer; dimer of dimers. {ECO:0000269\|PubMed:10527934, ECO:0000269\|PubMed:15265860, ECO:0000269\|Ref.5}.
Miscellaneous:		The metabolic pathway in Sulfolobus species contrasts with the situation observed in other microorganisms where separate enzymes exist for the catabolism of the two sugars, glucose and galactose. {ECO:0000305\|PubMed:12824170}.
Similarity:		Belongs to the DapA family. KDPG aldolase subfamily. {ECO:0000305}.