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UniProt functional annotation for Q9WWU5 | ||
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| UniProt code: Q9WWU5. |
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| Organism: | |
Pseudomonas putida (Arthrobacter siderocapsulatus). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales; Pseudomonadaceae; Pseudomonas. |
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| Function: | |
Catalyzes the oxygenolytic cleavage of 2-ethylhexyl sulfate (2-EHS) in the presence of alpha-ketoglutarate to yield 2-ethyl-hexanal and succinate, the decarboxylated form of alpha-ketoglutarate. It can accept a wide range of alpha-keto acids including 2-oxo-valerate, 2- oxo-adipate, 2-oxo-octanoate, 3-methyl-2-oxo-butyrate, oxaloacetate- alpha-ketoadipate, and alpha-ketooctanoate. It can catalyze the cleavage of medium-chain alkyl sulfate esters such as butylsulfate, pentylsulfate, hexylsulfate, heptylsulfate, octylsulfate, nonylsulfate, decylsulfate and sodium dodecyl sulfate (SDS). {ECO:0000269|PubMed:10913158}. |
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| Catalytic activity: | |
Reaction=2-ethylhexyl sulfate + 2-oxoglutarate + O2 = 2-ethylhexanal + CO2 + H(+) + succinate + sulfate; Xref=Rhea:RHEA:47620, ChEBI:CHEBI:15378, ChEBI:CHEBI:15379, ChEBI:CHEBI:16189, ChEBI:CHEBI:16526, ChEBI:CHEBI:16810, ChEBI:CHEBI:30031, ChEBI:CHEBI:87808, ChEBI:CHEBI:87809; Evidence={ECO:0000269|PubMed:10913158}; |
| Cofactor: | |
Name=Fe(2+); Xref=ChEBI:CHEBI:29033; Evidence={ECO:0000269|PubMed:10913158, ECO:0000269|PubMed:15023059, ECO:0000269|PubMed:15542595}; |
| Activity regulation: | |
Strongly stimulated by ascorbate. {ECO:0000269|PubMed:10913158}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=34 mM for SDS (at pH 7 and 30 degrees Celsius) {ECO:0000269|PubMed:10913158}; KM=40 mM for hexylsulfate (at pH 7 and 30 degrees Celsius) {ECO:0000269|PubMed:10913158}; KM=60 mM for nonylsulfate (at pH 7 and 30 degrees Celsius) {ECO:0000269|PubMed:10913158}; KM=66 mM for 2-EHS (at pH 7 and 30 degrees Celsius) {ECO:0000269|PubMed:10913158}; KM=97 mM for decylsulfate (at pH 7 and 30 degrees Celsius) {ECO:0000269|PubMed:10913158}; KM=140 mM for alpha-ketoglutarate (at pH 7 and 30 degrees Celsius) {ECO:0000269|PubMed:10913158}; KM=271 mM for heptylsulfate (at pH 7 and 30 degrees Celsius) {ECO:0000269|PubMed:10913158}; KM=437 mM for octylsulfate (at pH 7 and 30 degrees Celsius) {ECO:0000269|PubMed:10913158}; pH dependence: Optimum pH is 7. {ECO:0000269|PubMed:10913158}; |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:10913158, ECO:0000269|PubMed:15023059, ECO:0000269|PubMed:15542595}. |
| Similarity: | |
Belongs to the TfdA dioxygenase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.