PDBsum entry 1vm1

Hydrolase

PDB id: 1vm1

Contents

Protein chain

265 a.a. *

Ligands

MA4 ×2

TBE

AKR

TAZ

Waters ×175

* Residue conservation analysis

References listed in PDB file

Key reference

• Title: Inhibition of the shv-1 beta-Lactamase by sulfones: crystallographic observation of two reaction intermediates with tazobactam.
• Authors: A.P.Kuzin, M.Nukaga, Y.Nukaga, A.Hujer, R.A.Bonomo, J.R.Knox.
• Ref.: Biochemistry, 2001, 40, 1861-1866. [DOI no: 10.1021/bi0022745]
• PubMed id: 11327849

Abstract

Two species resulting from the reaction of the SHV-1 class A beta-lactamase with the sulfone inhibitor tazobactam have been trapped at 100 K and mapped by X-ray crystallography at 2.0 A resolution. An acyclic form of tazobactam is covalently bonded to the catalytic Ser70 side chain, and a second species, a five-atom vinyl carboxylic acid fragment of tazobactam, is bonded to Ser130. It is proposed that the electron density map of the crystal is a composite picture of two complexes, each with only a single bound species. It is estimated that the two complexes exist in the crystal in approximately equal populations. Results are discussed in relation to the mechanism-based inhibition of class A beta-lactamases by the similar inhibitors sulbactam and clavulanic acid.

Secondary reference #1

• Title: Structure of the shv-1 beta-Lactamase.
• Authors: A.P.Kuzin, M.Nukaga, Y.Nukaga, A.M.Hujer, R.A.Bonomo, J.R.Knox.
• Ref.: Biochemistry, 1999, 38, 5720-5727. [DOI no: 10.1021/bi990136d]
• PubMed id: 10231522