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UniProt functional annotation for Q04066 | ||
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| UniProt code: Q04066. |
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| Organism: | |
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast). |
| Taxonomy: | |
Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes; Saccharomycetales; Saccharomycetaceae; Saccharomyces. |
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| Function: | |
Catalyzes the hydrolysis of N-formyl-L-kynurenine to L- kynurenine, the second step in the kynurenine pathway of tryptophan degradation. Kynurenine may be further oxidized to nicotinic acid, NAD(H) and NADP(H). Required for elimination of toxic metabolites. {ECO:0000255|HAMAP-Rule:MF_03014, ECO:0000269|PubMed:18205391}. |
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| Catalytic activity: | |
Reaction=H2O + N-formyl-L-kynurenine = formate + H(+) + L-kynurenine; Xref=Rhea:RHEA:13009, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:15740, ChEBI:CHEBI:57959, ChEBI:CHEBI:58629; EC=3.5.1.9; Evidence={ECO:0000255|HAMAP-Rule:MF_03014, ECO:0000269|PubMed:18205391}; |
| Pathway: | |
Amino-acid degradation; L-tryptophan degradation via kynurenine pathway; L-kynurenine from L-tryptophan: step 2/2. {ECO:0000255|HAMAP-Rule:MF_03014, ECO:0000269|PubMed:18205391}. |
| Subunit: | |
Homodimer. {ECO:0000255|HAMAP-Rule:MF_03014}. |
| Domain: | |
The main chain amide nitrogen atoms of the second glycine and its adjacent residue in the HGGXW motif define the oxyanion hole, and stabilize the oxyanion that forms during the nucleophilic attack by the catalytic serine during substrate cleavage. {ECO:0000269|PubMed:18205391}. |
| Disruption phenotype: | |
Cells exhibit slow growth in the absence of nicotinate. {ECO:0000269|PubMed:18205391}. |
| Similarity: | |
Belongs to the kynurenine formamidase family. {ECO:0000255|HAMAP-Rule:MF_03014}. |
Annotations taken from UniProtKB at the EBI.