|
|
||
|
|
|
|
|
UniProt functional annotation for Q8LAH7 | ||
|
|
|
|
|
|
||
| UniProt code: Q8LAH7. |
|
||
| Organism: | |
Arabidopsis thaliana (Mouse-ear cress). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. |
|
||
|
||
| Function: | |
Specifically cleaves olefinic bonds in alpha,beta-unsaturated carbonyls and may be involved in detoxification or modification of these reactive compounds (PubMed:9346960, PubMed:10872231). May be involved in the biosynthesis or metabolism of oxylipin signaling molecules (Probable). In vitro, reduces 9R,13R-12-oxophytodienoic acid (9R,13R-OPDA) to 9R,13R-OPC-8:0, but only poorly 9S,13S-OPDA, the natural precursor of jasmonic acid (PubMed:10872231). Can detoxify the explosive 2,4,6-trinitrotoluene (TNT) in vitro and in vivo by catalyzing its nitroreduction to form hydroxylamino-dinitrotoluene (HADNT) (PubMed:19605548). {ECO:0000269|PubMed:10872231, ECO:0000269|PubMed:19605548, ECO:0000269|PubMed:9346960, ECO:0000305|PubMed:9346960}. |
|
||
| Catalytic activity: | |
Reaction=NADP(+) + OPC-8 = (10Z,15Z)-12-oxophytodienoate + H(+) + NADPH; Xref=Rhea:RHEA:21888, ChEBI:CHEBI:15378, ChEBI:CHEBI:15720, ChEBI:CHEBI:57411, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.3.1.42; Evidence={ECO:0000269|PubMed:10872231, ECO:0000269|PubMed:9346960}; |
| Cofactor: | |
Name=FMN; Xref=ChEBI:CHEBI:58210; Evidence={ECO:0000269|PubMed:10872231}; |
| Pathway: | |
Lipid metabolism; oxylipin biosynthesis. {ECO:0000305}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:12445129}. |
| Tissue specificity: | |
Mostly expressed in roots, also present in leaves, shoots and flowers. More abundant in cotyledons. In more details, expressed in peduncles, sepals, petals, around the abscission zone of siliques, maturing siliques and developing seeds. {ECO:0000269|PubMed:10333582}. |
| Developmental stage: | |
Expressed during leaves senescence, seeds development, and siliques maturation. |
| Induction: | |
By wounding, locally and systemically, by cold and heat stresses, by jasmonate and by UV-C. Up-regulated during senescence. Seems to not be influenced by UV-A and UV-B. Induced by the explosive 2,4,6-trinitrotoluene (TNT). {ECO:0000269|PubMed:10333582, ECO:0000269|PubMed:11725945, ECO:0000269|PubMed:19605548}. |
| Similarity: | |
Belongs to the NADH:flavin oxidoreductase/NADH oxidase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.