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UniProt functional annotation for Q8VDQ1 | ||
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| UniProt code: Q8VDQ1. |
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| Organism: | |
Mus musculus (Mouse). |
| Taxonomy: | |
Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus. |
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| Function: | |
Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-keto-PGE1, 15-keto-PGE2, 15-keto-PGE1-alpha and 15-keto-PGE2-alpha with highest activity towards 15-keto-PGE2. Overexpression represses transcriptional activity of PPARG and inhibits adipocyte differentiation. {ECO:0000269|PubMed:17449869}. |
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| Catalytic activity: | |
Reaction=13,14-dihydro-15-oxo-prostaglandin E2 + NAD(+) = 15- oxoprostaglandin E2 + H(+) + NADH; Xref=Rhea:RHEA:11916, ChEBI:CHEBI:15378, ChEBI:CHEBI:57400, ChEBI:CHEBI:57402, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.48; Evidence={ECO:0000269|PubMed:17449869}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:11918; Evidence={ECO:0000305|PubMed:17449869}; |
| Catalytic activity: | |
Reaction=13,14-dihydro-15-oxo-prostaglandin E2 + NADP(+) = 15- oxoprostaglandin E2 + H(+) + NADPH; Xref=Rhea:RHEA:11912, ChEBI:CHEBI:15378, ChEBI:CHEBI:57400, ChEBI:CHEBI:57402, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.3.1.48; Evidence={ECO:0000269|PubMed:17449869}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:11914; Evidence={ECO:0000305|PubMed:17449869}; |
| Catalytic activity: | |
Reaction=13,14-dihydro-15-oxo-PGF2alpha + NADP(+) = 15-oxoprostaglandin F2alpha + H(+) + NADPH; Xref=Rhea:RHEA:50588, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133374, ChEBI:CHEBI:133409; Evidence={ECO:0000269|PubMed:17449869}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50590; Evidence={ECO:0000305|PubMed:17449869}; |
| Catalytic activity: | |
Reaction=13,14-dihydro-15-oxo-prostaglandin E1 + NADP(+) = 15- oxoprostaglandin E1 + H(+) + NADPH; Xref=Rhea:RHEA:50584, ChEBI:CHEBI:15378, ChEBI:CHEBI:57401, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133408; Evidence={ECO:0000269|PubMed:17449869}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50586; Evidence={ECO:0000305|PubMed:17449869}; |
| Catalytic activity: | |
Reaction=13,14-dihydro-15-oxo-prostaglandin F1alpha + NADP(+) = 15- oxoprostaglandin F1alpha + H(+) + NADPH; Xref=Rhea:RHEA:50592, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:79072, ChEBI:CHEBI:133411; Evidence={ECO:0000269|PubMed:17449869}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50594; Evidence={ECO:0000305|PubMed:17449869}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=49.6 uM for 15-keto-PGE2 {ECO:0000269|PubMed:17449869}; KM=34.4 uM for 15-keto-PGE1 {ECO:0000269|PubMed:17449869}; KM=108.8 uM for 15-keto-PGF2-alpha {ECO:0000269|PubMed:17449869}; KM=59.2 uM for 15-keto-PGF2-beta {ECO:0000269|PubMed:17449869}; KM=94.6 uM for NADPH {ECO:0000269|PubMed:17449869}; Vmax=178.4 umol/min/mg enzyme for 15-keto-PGE2 {ECO:0000269|PubMed:17449869}; Vmax=115.0 umol/min/mg enzyme for 15-keto-PGE1 {ECO:0000269|PubMed:17449869}; Vmax=230.9 umol/min/mg enzyme for 15-keto-PGF2-alpha {ECO:0000269|PubMed:17449869}; Vmax=206.4 umol/min/mg enzyme for 15-keto-PGF2-beta {ECO:0000269|PubMed:17449869}; Vmax=144.7 umol/min/mg enzyme for NADPH {ECO:0000269|PubMed:17449869}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:15229897, ECO:0000269|PubMed:19000823}. |
| Subcellular location: | |
Cytoplasm {ECO:0000250}. |
| Tissue specificity: | |
Widely expressed with highest levels in adipose tissues. {ECO:0000269|PubMed:17449869}. |
| Developmental stage: | |
Highly expressed in the late phase of adipocyte differentiation (at protein level). {ECO:0000269|PubMed:17449869}. |
| Similarity: | |
Belongs to the NADP-dependent oxidoreductase L4BD family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.