|
|
||
|
|
|
|
|
UniProt functional annotation for P00383 | ||
|
|
|
|
|
|
||
| UniProt code: P00383. |
|
||
| Organism: | |
Escherichia coli. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
|
||
|
||
| Function: | |
Key enzyme in folate metabolism. Catalyzes an essential reaction for de novo glycine and purine synthesis, and for DNA precursor synthesis. |
|
||
| Catalytic activity: | |
Reaction=(6S)-5,6,7,8-tetrahydrofolate + NADP(+) = 7,8-dihydrofolate + H(+) + NADPH; Xref=Rhea:RHEA:15009, ChEBI:CHEBI:15378, ChEBI:CHEBI:57451, ChEBI:CHEBI:57453, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.5.1.3; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=5.8 uM for dihydrofolate {ECO:0000269|PubMed:11560482}; KM=3.0 uM for NADPH {ECO:0000269|PubMed:11560482}; |
| Pathway: | |
Cofactor biosynthesis; tetrahydrofolate biosynthesis; 5,6,7,8- tetrahydrofolate from 7,8-dihydrofolate: step 1/1. |
| Subunit: | |
Homotetramer. {ECO:0000269|PubMed:11560482, ECO:0000269|PubMed:16790925, ECO:0000269|PubMed:17473013, ECO:0000269|PubMed:18052202, ECO:0000269|PubMed:7583655}. |
| Domain: | |
The active site is situated at the inner surface of a pore formed by the four subunits. {ECO:0000269|PubMed:16790925, ECO:0000269|PubMed:7583655}. |
| Miscellaneous: | |
Type II plasmid-specified enzyme is practically insensitive to trimethoprim and methotrexate. |
Annotations taken from UniProtKB at the EBI.