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PDBsum entry 1vif
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Oxidoreductase
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PDB id
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1vif
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References listed in PDB file
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Key reference
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Title
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A plasmid-Encoded dihydrofolate reductase from trimethoprim-Resistant bacteria has a novel d2-Symmetric active site.
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Authors
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N.Narayana,
D.A.Matthews,
E.E.Howell,
X.Nguyen-Huu.
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Ref.
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Nat Struct Biol, 1995,
2,
1018-1025.
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PubMed id
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Abstract
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Bacteria expressing R67-plasmid encoded dihydrofolate reductase (R67 DHFR)
exhibit high-level resistance to the antibiotic trimethoprim. Native R67 DHFR is
a 34,000 M(r) homotetramer which exists in equilibrium with an inactive dimeric
form. The structure of native R67 DHFR has now been solved at 1.7 A resolution
and is unrelated to that of chromosomal DHFR. Homotetrameric R67 DHFR has an
unusual pore, 25 A in length, passing through the middle of the molecule. Two
folate molecules bind asymmetrically within the pore indicating that the
enzyme's active site consists of symmetry related binding surfaces from all four
identical units.
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Secondary reference #1
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Title
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Does r67 dihydrofolate reductase possess a proton donor?
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Authors
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J.C.Holland,
C.E.Linn,
E.Digiammarino,
R.Nichols,
E.E.Howell.
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Ref.
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Adv Exp Med Biol, 1993,
338,
493-498.
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PubMed id
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Secondary reference #2
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Title
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Construction of a synthetic gene for an r-Plasmid-Encoded dihydrofolate reductase and studies on the role of the n-Terminus in the protein.
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Authors
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L.J.Reece,
R.Nichols,
R.C.Ogden,
E.E.Howell.
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Ref.
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Biochemistry, 1991,
30,
10895-10904.
[DOI no: ]
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PubMed id
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Secondary reference #3
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Title
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Crystal structure of a novel trimethoprim-Resistant dihydrofolate reductase specified in escherichia coli by r-Plasmid r67.
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Authors
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D.A.Matthews,
S.L.Smith,
D.P.Baccanari,
J.J.Burchall,
S.J.Oatley,
J.Kraut.
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Ref.
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Biochemistry, 1986,
25,
4194-4204.
[DOI no: ]
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PubMed id
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Secondary reference #4
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Title
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The amino acid sequence of the trimethoprim-Resistant dihydrofolate reductase specified in escherichia coli by r-Plasmid r67.
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Authors
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D.Stone,
S.L.Smith.
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Ref.
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J Biol Chem, 1979,
254,
10857-10861.
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PubMed id
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Secondary reference #5
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Title
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Trimethoprim resistance determined by r factors.
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Authors
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M.P.Fleming,
N.Datta,
R.N.Grüneberg.
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Ref.
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Br Med J, 1972,
1,
726-728.
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PubMed id
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