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UniProt functional annotation for P0A6I9 | ||
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| UniProt code: P0A6I9. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Catalyzes the phosphorylation of the 3'-hydroxyl group of dephosphocoenzyme A to form coenzyme A. {ECO:0000255|HAMAP- Rule:MF_00376, ECO:0000269|PubMed:11292795}. |
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| Catalytic activity: | |
Reaction=3'-dephospho-CoA + ATP = ADP + CoA + H(+); Xref=Rhea:RHEA:18245, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57287, ChEBI:CHEBI:57328, ChEBI:CHEBI:456216; EC=2.7.1.24; Evidence={ECO:0000255|HAMAP-Rule:MF_00376, ECO:0000269|PubMed:11292795}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.74 mM for dephospho-CoA {ECO:0000269|PubMed:11292795}; KM=0.14 mM for ATP {ECO:0000269|PubMed:11292795}; pH dependence: Optimum pH is 8.5. {ECO:0000269|PubMed:11292795}; |
| Pathway: | |
Cofactor biosynthesis; coenzyme A biosynthesis; CoA from (R)- pantothenate: step 5/5. {ECO:0000255|HAMAP-Rule:MF_00376, ECO:0000305|PubMed:11292795}. |
| Subunit: | |
Monomer (PubMed:11292795, PubMed:12538896). Forms homotrimers in the presence of sulfate. The trimeric form exists in solution in equilibrium with the monomeric form (PubMed:12538896). {ECO:0000269|PubMed:11292795, ECO:0000269|PubMed:12538896}. |
| Subcellular location: | |
Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00376, ECO:0000305}. |
| Similarity: | |
Belongs to the CoaE family. {ECO:0000255|HAMAP- Rule:MF_00376, ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.