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UniProt functional annotation for P52699 | ||
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| UniProt code: P52699. |
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| Organism: | |
Serratia marcescens. |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Yersiniaceae; Serratia. |
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| Function: | |
Confers resistance to the different beta-lactams antibiotics (penicillin, cephalosporin and carbapenem) via the hydrolysis of the beta-lactam ring. {ECO:0000269|PubMed:8141584}. |
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| Catalytic activity: | |
Reaction=a beta-lactam + H2O = a substituted beta-amino acid; Xref=Rhea:RHEA:20401, ChEBI:CHEBI:15377, ChEBI:CHEBI:35627, ChEBI:CHEBI:140347; EC=3.5.2.6; Evidence={ECO:0000269|PubMed:8141584}; |
| Cofactor: | |
Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:26482303}; Note=Binds 2 Zn(2+) ions per subunit. {ECO:0000269|PubMed:26482303}; |
| Activity regulation: | |
Inhibited by captopril stereoisomers, Hg(2+), Fe(2+), Cu(2+) and by chelating agents such as EDTA (PubMed:8141584, PubMed:26482303). This enzyme is not susceptible to inactivation by the beta-lactamase-blocking agents clavulanic acid or cloxacillin (PubMed:8141584). {ECO:0000269|PubMed:26482303, ECO:0000269|PubMed:8141584}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.74 uM for meropenem {ECO:0000269|PubMed:8141584}; KM=1.24 uM for ceftazidime {ECO:0000269|PubMed:8141584}; KM=2.07 uM for ceftizoxime and panipenem {ECO:0000269|PubMed:8141584}; KM=2.13 uM for cefoperazone {ECO:0000269|PubMed:8141584}; KM=2.15 uM for ampicillin {ECO:0000269|PubMed:8141584}; KM=3.97 uM for aztreonam {ECO:0000269|PubMed:8141584}; KM=7.33 uM for imipenem {ECO:0000269|PubMed:8141584}; KM=7.55 uM for moxalactam {ECO:0000269|PubMed:8141584}; KM=7.74 uM for cephaloridine {ECO:0000269|PubMed:8141584}; |
| Subunit: | |
Monomer. {ECO:0000250|UniProtKB:P25910}. |
| Subcellular location: | |
Periplasm {ECO:0000305}. |
| Similarity: | |
Belongs to the metallo-beta-lactamase superfamily. Class-B beta-lactamase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.