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UniProt functional annotation for Q75UV1 | ||
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| UniProt code: Q75UV1. |
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| Organism: | |
Thermus thermophilus. |
| Taxonomy: | |
Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus. |
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| Function: | |
Specifically hydrolyzes (di)adenosine polyphosphates but not ATP or diadenosine triphosphate, generating ATP as the product. Diadenosine hexaphosphate (Ap6A) is the preferred substrate and hydrolysis yields 2 ATP. It is the only enzyme that symmetrically hydrolyzes Ap6A. It also hydrolyzes diadenosine pentaphosphate (Ap5A), diadenosine tetraphosphate (Ap4A) and adenosine tetraphosphate (p4A). {ECO:0000269|PubMed:15024014}. |
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| Catalytic activity: | |
Reaction=H2O + P(1),P(6)-bis(5'-adenosyl) hexaphosphate = 2 ATP + 2 H(+); Xref=Rhea:RHEA:32043, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:63740; EC=3.6.1.61; Evidence={ECO:0000269|PubMed:15024014}; |
| Catalytic activity: | |
Reaction=H2O + P(1),P(5)-bis(5'-adenosyl) pentaphosphate = ADP + ATP + 2 H(+); Xref=Rhea:RHEA:30527, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:62041, ChEBI:CHEBI:456216; EC=3.6.1.61; Evidence={ECO:0000269|PubMed:15024014}; |
| Catalytic activity: | |
Reaction=H2O + P(1),P(4)-bis(5'-adenosyl) tetraphosphate = AMP + ATP + 2 H(+); Xref=Rhea:RHEA:32039, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:58141, ChEBI:CHEBI:456215; EC=3.6.1.61; Evidence={ECO:0000269|PubMed:15024014}; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:15024014}; Note=Also able to use Mn(2+) and Zn(2+), but the activity is less than that obtained with Mg(2+) ions. {ECO:0000269|PubMed:15024014}; |
| Activity regulation: | |
Strongly inhibited by fluoride ions. {ECO:0000269|PubMed:15024014}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1 uM for p4A (at 25 degrees Celsius) {ECO:0000269|PubMed:15024014}; KM=1.1 uM for Ap4A (at 25 degrees Celsius) {ECO:0000269|PubMed:15024014}; KM=1.1 uM for Ap5A (at 25 degrees Celsius) {ECO:0000269|PubMed:15024014}; KM=1.4 uM for Ap6A (at 25 degrees Celsius) {ECO:0000269|PubMed:15024014}; KM=1.4 uM for diguanosine pentaphosphate (Gp5G) (at 25 degrees Celsius) {ECO:0000269|PubMed:15024014}; KM=9.3 uM for diguanosine tetraphosphate (Gp4G) (at 25 degrees Celsius) {ECO:0000269|PubMed:15024014}; Note=Kcat is 4.1 sec(-1) with Ap6A (at 25 degrees Celsius). Kcat is 1.4 sec(-1) with p4A (at 25 degrees Celsius). Kcat is 0.52 sec(-1) with Ap5A (at 25 degrees Celsius). Kcat is 0.27 sec(-1) with Ap4A (at 25 degrees Celsius).; pH dependence: Optimum pH is 8. {ECO:0000269|PubMed:15024014}; Temperature dependence: Optimum temperature is 70 degrees Celsius. Ndx1 is stable up to 95 degrees Celsius at pH 7.5. {ECO:0000269|PubMed:15024014}; |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:15024014, ECO:0000305|Ref.2}. |
| Similarity: | |
Belongs to the Nudix hydrolase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.