UniProt functional annotation for P68698



	UniProt functional annotation for P68698

UniProt code: P68698.

Organism: Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)).

Taxonomy: Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.

Function: Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. {ECO:0000269|PubMed:16669621, ECO:0000269|PubMed:20187656}.

Catalytic activity: Reaction=ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.; EC=5.6.2.1; Evidence={ECO:0000255|PROSITE- ProRule:PRU10130};

Subcellular location: Virion {ECO:0000305}.

Induction: Expressed in the late phase of the viral replicative cycle.

Similarity: Belongs to the type IB topoisomerase family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Vaccinia virus (strain Western Reserve) (VACV) (Vaccinia virus (strain WR)).
Taxonomy:		Viruses; Varidnaviria; Bamfordvirae; Nucleocytoviricota; Pokkesviricetes; Chitovirales; Poxviridae; Chordopoxvirinae; Orthopoxvirus; Vaccinia virus.



Function:		Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at the specific target site 5'-[CT]CCTTp site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(3'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 5'-OH DNA strand. The free DNA strand then undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 5'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone. {ECO:0000269\|PubMed:16669621, ECO:0000269\|PubMed:20187656}.


Catalytic activity:		Reaction=ATP-independent breakage of single-stranded DNA, followed by passage and rejoining.; EC=5.6.2.1; Evidence={ECO:0000255\|PROSITE- ProRule:PRU10130};
Subcellular location:		Virion {ECO:0000305}.
Induction:		Expressed in the late phase of the viral replicative cycle.
Similarity:		Belongs to the type IB topoisomerase family. {ECO:0000305}.