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UniProt functional annotation for Q9GV41 | ||
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| UniProt code: Q9GV41. |
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| Organism: | |
Trypanosoma brucei brucei. |
| Taxonomy: | |
Eukaryota; Discoba; Euglenozoa; Kinetoplastea; Metakinetoplastina; Trypanosomatida; Trypanosomatidae; Trypanosoma. |
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| Function: | |
Catalyzes the NADP-dependent formation of prostaglandin F2- alpha from prostaglandin H2. Has also aldo/ketoreductase activity towards the synthetic substrates 9,10-phenanthrenequinone and p- nitrobenzaldehyde. {ECO:0000269|PubMed:11067881}. |
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| Catalytic activity: | |
Reaction=NADP(+) + prostaglandin F2alpha = H(+) + NADPH + prostaglandin H2; Xref=Rhea:RHEA:45312, ChEBI:CHEBI:15378, ChEBI:CHEBI:57404, ChEBI:CHEBI:57405, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; Evidence={ECO:0000269|PubMed:11067881}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.3 uM for prostaglandin H2 {ECO:0000269|PubMed:11067881}; KM=0.42 uM for 9,10-phenanthrenequinone {ECO:0000269|PubMed:11067881}; KM=0.8 uM for p-nitrobenzaldehyde {ECO:0000269|PubMed:11067881}; KM=5.7 uM for NADPH {ECO:0000269|PubMed:11067881}; Vmax=2 umol/min/mg enzyme toward prostaglandin H2 {ECO:0000269|PubMed:11067881}; Vmax=22 umol/min/mg enzyme toward p-nitrobenzaldehyde {ECO:0000269|PubMed:11067881}; Vmax=48 umol/min/mg enzyme toward 9,10-phenanthrenequinone {ECO:0000269|PubMed:11067881}; pH dependence: Optimum pH is 6-9. {ECO:0000269|PubMed:11067881}; |
| Pathway: | |
Lipid metabolism; prostaglandin biosynthesis. |
| Subunit: | |
Monomer. {ECO:0000269|PubMed:11067881, ECO:0000269|Ref.2}. |
| Subcellular location: | |
Cytoplasm {ECO:0000269|PubMed:11067881}. |
| Similarity: | |
Belongs to the aldo/keto reductase family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.