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UniProt functional annotation for Q76IQ9 | ||
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| UniProt code: Q76IQ9. |
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| Organism: | |
Vibrio proteolyticus (Aeromonas proteolytica). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae; Vibrio. |
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| Function: | |
Catalyzes the reversible phosphorolysis of chitobiose (N,N'- diacetylchitobiose or (GlcNAc)(2)) into alpha-GlcNAc-1-phosphate and GlcNAc with inversion of the anomeric configuration. {ECO:0000269|PubMed:13678418}. |
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| Catalytic activity: | |
Reaction=N,N'-diacetylchitobiose + phosphate = N-acetyl-alpha-D- glucosamine 1-phosphate + N-acetyl-D-glucosamine; Xref=Rhea:RHEA:32527, ChEBI:CHEBI:28681, ChEBI:CHEBI:43474, ChEBI:CHEBI:57776, ChEBI:CHEBI:506227; EC=2.4.1.280; Evidence={ECO:0000269|PubMed:13678418}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=2.0 mM for N,N'-diacetylchitobiose {ECO:0000269|PubMed:13678418}; KM=69 mM for alpha-D-glucosyl-(1-4)-2-acetamide-2-deoxy-D-glucose {ECO:0000269|PubMed:13678418}; KM=14 mM for N-acetyl-alpha-D-glucosamine 1-phosphate {ECO:0000269|PubMed:13678418}; KM=0.48 mM for alpha-D-glucose 1-phosphate {ECO:0000269|PubMed:13678418}; pH dependence: Optimum pH is 7.0-7.7. {ECO:0000269|PubMed:13678418}; Temperature dependence: Optimum temperature is 30 degrees Celsius. {ECO:0000269|PubMed:13678418}; |
| Subunit: | |
Homodimer. {ECO:0000269|PubMed:15274915}. |
| Similarity: | |
Belongs to the glycosyl hydrolase 94 family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.