UniProt functional annotation for Q9D1E6



	UniProt functional annotation for Q9D1E6

UniProt code: Q9D1E6.

Organism: Mus musculus (Mouse).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.

Function: Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer (By similarity). Involved in regulation of tubulin heterodimer dissociation (PubMed:17184771). May function as a negative regulator of axonal growth (PubMed:17217416). {ECO:0000250|UniProtKB:Q99426, ECO:0000269|PubMed:17184771, ECO:0000269|PubMed:17217416}.

Subunit: Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state (By similarity). Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers (PubMed:17184771). Interacts with GAN. Interacts with DCTN1 (By similarity). {ECO:0000250|UniProtKB:Q99426, ECO:0000269|PubMed:17184771}.

Subcellular location: Cytoplasm {ECO:0000269|PubMed:17217416}. Cytoplasm, cytoskeleton {ECO:0000269|PubMed:17217416}. Note=Colocalizes with microtubules. In differentiated neurons, located in the cytoplasm. In differentiating neurons, accumulates at the growth cone. {ECO:0000269|PubMed:17217416}.

Tissue specificity: Widely expressed with highest levels in brain. Broadly distributed throughout the neonate brain but restricted mainly to ependymary cells in the adult brain where it is concentrated in the cilia. {ECO:0000269|PubMed:17217416}.

Developmental stage: In the embryo, expression increases at 12.5 dpc- 14.5 dpc. Levels are highest in pre- and postnatal stages which coincide with peaks of cerebral and cerebellar neurogenesis (at protein level). {ECO:0000269|PubMed:17217416}.

Ptm: Phosphorylation by PAK1 is required for normal function. {ECO:0000250|UniProtKB:Q99426}.

Ptm: Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome. {ECO:0000250|UniProtKB:Q99426}.

Disruption phenotype: Mice display significantly longer axons than wild-type mice. Overexpression of Tbcb causes microtubule depolymerization, growth cone retraction and axonal damage followed by neuronal degeneration. {ECO:0000269|PubMed:17217416}.

Similarity: Belongs to the TBCB family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Mus musculus (Mouse).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; Murinae; Mus; Mus.



Function:		Binds to alpha-tubulin folding intermediates after their interaction with cytosolic chaperonin in the pathway leading from newly synthesized tubulin to properly folded heterodimer (By similarity). Involved in regulation of tubulin heterodimer dissociation (PubMed:17184771). May function as a negative regulator of axonal growth (PubMed:17217416). {ECO:0000250\|UniProtKB:Q99426, ECO:0000269\|PubMed:17184771, ECO:0000269\|PubMed:17217416}.


Subunit:		Supercomplex made of cofactors A to E. Cofactors A and D function by capturing and stabilizing tubulin in a quasi-native conformation. Cofactor E binds to the cofactor D-tubulin complex; interaction with cofactor C then causes the release of tubulin polypeptides that are committed to the native state (By similarity). Cofactors B and E can form a heterodimer which binds to alpha-tubulin and enhances their ability to dissociate tubulin heterodimers (PubMed:17184771). Interacts with GAN. Interacts with DCTN1 (By similarity). {ECO:0000250\|UniProtKB:Q99426, ECO:0000269\|PubMed:17184771}.
Subcellular location:		Cytoplasm {ECO:0000269\|PubMed:17217416}. Cytoplasm, cytoskeleton {ECO:0000269\|PubMed:17217416}. Note=Colocalizes with microtubules. In differentiated neurons, located in the cytoplasm. In differentiating neurons, accumulates at the growth cone. {ECO:0000269\|PubMed:17217416}.
Tissue specificity:		Widely expressed with highest levels in brain. Broadly distributed throughout the neonate brain but restricted mainly to ependymary cells in the adult brain where it is concentrated in the cilia. {ECO:0000269\|PubMed:17217416}.
Developmental stage:		In the embryo, expression increases at 12.5 dpc- 14.5 dpc. Levels are highest in pre- and postnatal stages which coincide with peaks of cerebral and cerebellar neurogenesis (at protein level). {ECO:0000269\|PubMed:17217416}.
Ptm:		Phosphorylation by PAK1 is required for normal function. {ECO:0000250\|UniProtKB:Q99426}.
Ptm:		Ubiquitinated in the presence of GAN which targets it for degradation by the proteasome. {ECO:0000250\|UniProtKB:Q99426}.
Disruption phenotype:		Mice display significantly longer axons than wild-type mice. Overexpression of Tbcb causes microtubule depolymerization, growth cone retraction and axonal damage followed by neuronal degeneration. {ECO:0000269\|PubMed:17217416}.
Similarity:		Belongs to the TBCB family. {ECO:0000305}.