 |
PDBsum entry 1v41
|
|
|
|
References listed in PDB file
|
 |
|
Key reference
|
|
|
Title
|
|
Structure of human pnp complexed with ligands.
|
|
|
Authors
|
|
F.Canduri,
R.G.Silva,
D.M.Dos santos,
M.S.Palma,
L.A.Basso,
D.S.Santos,
W.F.De azevedo.
|
|
|
Ref.
|
|
Acta Crystallogr D Biol Crystallogr, 2005,
61,
856-862.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
Abstract
|
|
|
Purine nucleoside phosphorylase (PNP) is a key enzyme in the purine-salvage
pathway, which allows cells to utilize preformed bases and nucleosides in order
to synthesize nucleotides. PNP is specific for purine nucleosides in the
beta-configuration and exhibits a strong preference for purines containing a
6-keto group and ribosyl-containing nucleosides relative to the corresponding
analogues. PNP was crystallized in complex with ligands and data collection was
performed using synchrotron radiation. This work reports the structure of human
PNP in complex with guanosine (at 2.80 A resolution), 3'-deoxyguanosine (at 2.86
A resolution) and 8-azaguanine (at 2.85 A resolution). These structures were
compared with the PNP-guanine, PNP-inosine and PNP-immucillin-H complexes solved
previously.
|
|
|
|
|
|
|
|
Figure 3.
Figure 3 Superposition of PNP apoenzyme with the PNP-guanine
complex, showing the gate movement, which involves a helical
transformation of residues 257-265 in the transition apoenzyme
complex. Movements of the residues Tyr249, His257 and Lys265 and
the position of ligand and sulfates can be observed. The figure
was generated with the program MOLMOL (Koradi et al.,
1996[Koradi, R., Billeter, M. & Wuthrich, K. (1996). J. Mol.
Graph. 14, 51-55.]).
|
|
Figure 4.
Figure 4 Binding site of human PNP. (a) HsPNP-guanosine, (b)
HsPNP-inosine, (c) HsPNP-immucillin-H, (d)
HsPNP-3'-deoxyguanosine, (e) HsPNP-8-azaguanine, (f)
HsPNP-guanine.
|
|
|
|
|
|
The above figures are
reprinted
by permission from the IUCr:
Acta Crystallogr D Biol Crystallogr
(2005,
61,
856-862)
copyright 2005.
|
|
|
Secondary reference #1
|
|
|
Title
|
|
Crystal structure of human purine nucleoside phosphorylase at 2.3a resolution.
|
|
|
Authors
|
|
W.F.De azevedo,
F.Canduri,
D.M.Dos santos,
R.G.Silva,
J.S.De oliveira,
L.P.De carvalho,
L.A.Basso,
M.A.Mendes,
M.S.Palma,
D.S.Santos.
|
|
|
Ref.
|
|
Biochem Biophys Res Commun, 2003,
308,
545-552.
[DOI no: ]
|
|
|
PubMed id
|
|
|
|
|
|
|
|
|
|
|
|
|
