UniProt functional annotation for Q9EQZ5



	UniProt functional annotation for Q9EQZ5

UniProt code: Q9EQZ5.

Organism: Cavia porcellus (Guinea pig).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae; Cavia.

Function: NAD(P)H-dependent oxidoreductase involved in metabolic inactivation of pro- and anti-inflammatory eicosanoids: prostaglandins (PG), leukotrienes (LT) and lipoxins (LX) (PubMed:11733004). Catalyzes with high efficiency the reduction of the 13,14 double bond of 15- oxoPGs, including 15-oxo-PGE1, 15-oxo-PGE2, 15-oxo-PGF1-alpha and 15- oxo-PGF2-alpha (PubMed:11733004) (By similarity). Catalyzes with lower efficiency the oxidation of the hydroxyl group at C12 of LTB4 and its derivatives, converting them into biologically less active 12-oxo-LTB4 metabolites (PubMed:11733004) (By similarity). Reduces 15-oxo-LXA4 to 13,14 dihydro-15-oxo-LXA4, enhancing neutrophil recruitment at the inflammatory site (By similarity). Plays a role in metabolic detoxification of alkenals and ketones. Reduces alpha,beta-unsaturated alkenals and ketones, particularly those with medium-chain length, showing highest affinity toward (2E)-decenal and (3E)-3-nonen-2-one (By similarity). May inactivate 4-hydroxy-2-nonenal, a cytotoxic lipid constituent of oxidized low-density lipoprotein particles (By similarity). {ECO:0000250|UniProtKB:P97584, ECO:0000250|UniProtKB:Q14914, ECO:0000250|UniProtKB:Q29073, ECO:0000269|PubMed:11733004}.

Catalytic activity: Reaction=13,14-dihydro-15-oxo-prostaglandin E1 + NADP(+) = 15- oxoprostaglandin E1 + H(+) + NADPH; Xref=Rhea:RHEA:50584, ChEBI:CHEBI:15378, ChEBI:CHEBI:57401, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133408; Evidence={ECO:0000250|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50586; Evidence={ECO:0000250|UniProtKB:Q14914};

Catalytic activity: Reaction=13,14-dihydro-15-oxo-prostaglandin E2 + NAD(+) = 15- oxoprostaglandin E2 + H(+) + NADH; Xref=Rhea:RHEA:11916, ChEBI:CHEBI:15378, ChEBI:CHEBI:57400, ChEBI:CHEBI:57402, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.48; Evidence={ECO:0000269|PubMed:11733004}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:11918; Evidence={ECO:0000305|PubMed:11733004};

Catalytic activity: Reaction=13,14-dihydro-15-oxo-prostaglandin F1alpha + NADP(+) = 15- oxoprostaglandin F1alpha + H(+) + NADPH; Xref=Rhea:RHEA:50592, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:79072, ChEBI:CHEBI:133411; Evidence={ECO:0000250|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50594; Evidence={ECO:0000250|UniProtKB:Q14914};

Catalytic activity: Reaction=13,14-dihydro-15-oxo-PGF2alpha + NADP(+) = 15-oxoprostaglandin F2alpha + H(+) + NADPH; Xref=Rhea:RHEA:50588, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133374, ChEBI:CHEBI:133409; Evidence={ECO:0000250|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50590; Evidence={ECO:0000250|UniProtKB:Q14914};

Catalytic activity: Reaction=leukotriene B4 + NADP(+) = 12-oxo-leukotriene B4 + H(+) + NADPH; Xref=Rhea:RHEA:50608, ChEBI:CHEBI:15378, ChEBI:CHEBI:57461, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133309; Evidence={ECO:0000269|PubMed:11733004}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50609; Evidence={ECO:0000305|PubMed:11733004};

Catalytic activity: Reaction=20-hydroxy-leukotriene B4 + NADP(+) = 12-oxo-20-hydroxy- leukotriene B4 + H(+) + NADPH; Xref=Rhea:RHEA:51208, ChEBI:CHEBI:15378, ChEBI:CHEBI:57460, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133346; Evidence={ECO:0000250|UniProtKB:Q29073}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51209; Evidence={ECO:0000250|UniProtKB:Q29073};

Catalytic activity: Reaction=6-trans-leukotriene B4 + NADP(+) = 12-oxo-(5S)-hydroxy- (6E,8E,10E,14Z)-eicosatetraenoate + H(+) + NADPH; Xref=Rhea:RHEA:51204, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:90723, ChEBI:CHEBI:133974; Evidence={ECO:0000250|UniProtKB:Q29073}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51205; Evidence={ECO:0000250|UniProtKB:Q29073};

Catalytic activity: Reaction=(5S,12S)-dihydroxy-(6E,10E,12E,14Z)-eicosatetraenoate + NADP(+) = 12-oxo-(5S)-hydroxy-(6E,8E,10E,14Z)-eicosatetraenoate + H(+) + NADPH; Xref=Rhea:RHEA:51212, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133974, ChEBI:CHEBI:133975; Evidence={ECO:0000250|UniProtKB:Q29073}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51213; Evidence={ECO:0000250|UniProtKB:Q29073};

Catalytic activity: Reaction=an n-alkanal + NADP(+) = an alk-2-enal + H(+) + NADPH; Xref=Rhea:RHEA:13737, ChEBI:CHEBI:12834, ChEBI:CHEBI:13757, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.3.1.74; Evidence={ECO:0000250|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:13739; Evidence={ECO:0000250|UniProtKB:Q14914};

Catalytic activity: Reaction=hexanal + NADP(+) = (E)-hex--2-enal + H(+) + NADPH; Xref=Rhea:RHEA:50776, ChEBI:CHEBI:15378, ChEBI:CHEBI:28913, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:88528; Evidence={ECO:0000250|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50778; Evidence={ECO:0000250|UniProtKB:Q14914};

Catalytic activity: Reaction=NADP(+) + octanal = (2E)-octenal + H(+) + NADPH; Xref=Rhea:RHEA:50780, ChEBI:CHEBI:15378, ChEBI:CHEBI:17935, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:61748; Evidence={ECO:0000250|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50782; Evidence={ECO:0000250|UniProtKB:Q14914};

Catalytic activity: Reaction=decanal + NADP(+) = (2E)-decenal + H(+) + NADPH; Xref=Rhea:RHEA:50612, ChEBI:CHEBI:15378, ChEBI:CHEBI:31457, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133455; Evidence={ECO:0000250|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50614; Evidence={ECO:0000250|UniProtKB:Q14914};

Catalytic activity: Reaction=dodecanal + NADP(+) = (2E)-dodecenal + H(+) + NADPH; Xref=Rhea:RHEA:50784, ChEBI:CHEBI:15378, ChEBI:CHEBI:27836, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133741; Evidence={ECO:0000250|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50786; Evidence={ECO:0000250|UniProtKB:Q14914};

Catalytic activity: Reaction=4-hydroxynonanal + NADP(+) = (E)-4-hydroxynon-2-enal + H(+) + NADPH; Xref=Rhea:RHEA:64736, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:58968, ChEBI:CHEBI:156112; Evidence={ECO:0000250|UniProtKB:P97584}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:64738; Evidence={ECO:0000250|UniProtKB:P97584};

Catalytic activity: Reaction=NADP(+) + pentan-2-one = (E)-pent-3-en-2-one + H(+) + NADPH; Xref=Rhea:RHEA:50788, ChEBI:CHEBI:15378, ChEBI:CHEBI:16472, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:145276; Evidence={ECO:0000250|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50790; Evidence={ECO:0000250|UniProtKB:Q14914};

Catalytic activity: Reaction=NADP(+) + nonan-2-one = (3E)-nonen-2-one + H(+) + NADPH; Xref=Rhea:RHEA:50616, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:77927, ChEBI:CHEBI:133457; Evidence={ECO:0000250|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50618; Evidence={ECO:0000250|UniProtKB:Q14914};

Biophysicochemical properties: Kinetic parameters: KM=93 uM for LTB4 {ECO:0000269|PubMed:11733004}; KM=35 uM for 15-keto-PGE2 {ECO:0000269|PubMed:11733004}; Vmax=1.7 nmol/min/mg enzyme with LTB4 as substrate {ECO:0000269|PubMed:11733004}; Vmax=345 nmol/min/mg enzyme with 15-keto-PGE2 as substrate {ECO:0000269|PubMed:11733004};

Subunit: Monomer or homodimer. {ECO:0000269|PubMed:15007077}.

Subcellular location: Cytoplasm {ECO:0000269|PubMed:11733004}.

Tissue specificity: Detected in small intestine, kidney, liver, spleen and stomach (at protein level). Detected in small intestine, kidney and liver. {ECO:0000269|PubMed:11733004}.

Similarity: Belongs to the NADP-dependent oxidoreductase L4BD family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Cavia porcellus (Guinea pig).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Glires; Rodentia; Hystricomorpha; Caviidae; Cavia.



Function:		NAD(P)H-dependent oxidoreductase involved in metabolic inactivation of pro- and anti-inflammatory eicosanoids: prostaglandins (PG), leukotrienes (LT) and lipoxins (LX) (PubMed:11733004). Catalyzes with high efficiency the reduction of the 13,14 double bond of 15- oxoPGs, including 15-oxo-PGE1, 15-oxo-PGE2, 15-oxo-PGF1-alpha and 15- oxo-PGF2-alpha (PubMed:11733004) (By similarity). Catalyzes with lower efficiency the oxidation of the hydroxyl group at C12 of LTB4 and its derivatives, converting them into biologically less active 12-oxo-LTB4 metabolites (PubMed:11733004) (By similarity). Reduces 15-oxo-LXA4 to 13,14 dihydro-15-oxo-LXA4, enhancing neutrophil recruitment at the inflammatory site (By similarity). Plays a role in metabolic detoxification of alkenals and ketones. Reduces alpha,beta-unsaturated alkenals and ketones, particularly those with medium-chain length, showing highest affinity toward (2E)-decenal and (3E)-3-nonen-2-one (By similarity). May inactivate 4-hydroxy-2-nonenal, a cytotoxic lipid constituent of oxidized low-density lipoprotein particles (By similarity). {ECO:0000250\|UniProtKB:P97584, ECO:0000250\|UniProtKB:Q14914, ECO:0000250\|UniProtKB:Q29073, ECO:0000269\|PubMed:11733004}.


Catalytic activity:		Reaction=13,14-dihydro-15-oxo-prostaglandin E1 + NADP(+) = 15- oxoprostaglandin E1 + H(+) + NADPH; Xref=Rhea:RHEA:50584, ChEBI:CHEBI:15378, ChEBI:CHEBI:57401, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133408; Evidence={ECO:0000250\|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50586; Evidence={ECO:0000250\|UniProtKB:Q14914};
Catalytic activity:		Reaction=13,14-dihydro-15-oxo-prostaglandin E2 + NAD(+) = 15- oxoprostaglandin E2 + H(+) + NADH; Xref=Rhea:RHEA:11916, ChEBI:CHEBI:15378, ChEBI:CHEBI:57400, ChEBI:CHEBI:57402, ChEBI:CHEBI:57540, ChEBI:CHEBI:57945; EC=1.3.1.48; Evidence={ECO:0000269\|PubMed:11733004}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:11918; Evidence={ECO:0000305\|PubMed:11733004};
Catalytic activity:		Reaction=13,14-dihydro-15-oxo-prostaglandin F1alpha + NADP(+) = 15- oxoprostaglandin F1alpha + H(+) + NADPH; Xref=Rhea:RHEA:50592, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:79072, ChEBI:CHEBI:133411; Evidence={ECO:0000250\|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50594; Evidence={ECO:0000250\|UniProtKB:Q14914};
Catalytic activity:		Reaction=13,14-dihydro-15-oxo-PGF2alpha + NADP(+) = 15-oxoprostaglandin F2alpha + H(+) + NADPH; Xref=Rhea:RHEA:50588, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133374, ChEBI:CHEBI:133409; Evidence={ECO:0000250\|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50590; Evidence={ECO:0000250\|UniProtKB:Q14914};
Catalytic activity:		Reaction=leukotriene B4 + NADP(+) = 12-oxo-leukotriene B4 + H(+) + NADPH; Xref=Rhea:RHEA:50608, ChEBI:CHEBI:15378, ChEBI:CHEBI:57461, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133309; Evidence={ECO:0000269\|PubMed:11733004}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:50609; Evidence={ECO:0000305\|PubMed:11733004};
Catalytic activity:		Reaction=20-hydroxy-leukotriene B4 + NADP(+) = 12-oxo-20-hydroxy- leukotriene B4 + H(+) + NADPH; Xref=Rhea:RHEA:51208, ChEBI:CHEBI:15378, ChEBI:CHEBI:57460, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133346; Evidence={ECO:0000250\|UniProtKB:Q29073}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51209; Evidence={ECO:0000250\|UniProtKB:Q29073};
Catalytic activity:		Reaction=6-trans-leukotriene B4 + NADP(+) = 12-oxo-(5S)-hydroxy- (6E,8E,10E,14Z)-eicosatetraenoate + H(+) + NADPH; Xref=Rhea:RHEA:51204, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:90723, ChEBI:CHEBI:133974; Evidence={ECO:0000250\|UniProtKB:Q29073}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51205; Evidence={ECO:0000250\|UniProtKB:Q29073};
Catalytic activity:		Reaction=(5S,12S)-dihydroxy-(6E,10E,12E,14Z)-eicosatetraenoate + NADP(+) = 12-oxo-(5S)-hydroxy-(6E,8E,10E,14Z)-eicosatetraenoate + H(+) + NADPH; Xref=Rhea:RHEA:51212, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133974, ChEBI:CHEBI:133975; Evidence={ECO:0000250\|UniProtKB:Q29073}; PhysiologicalDirection=left-to-right; Xref=Rhea:RHEA:51213; Evidence={ECO:0000250\|UniProtKB:Q29073};
Catalytic activity:		Reaction=an n-alkanal + NADP(+) = an alk-2-enal + H(+) + NADPH; Xref=Rhea:RHEA:13737, ChEBI:CHEBI:12834, ChEBI:CHEBI:13757, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349; EC=1.3.1.74; Evidence={ECO:0000250\|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:13739; Evidence={ECO:0000250\|UniProtKB:Q14914};
Catalytic activity:		Reaction=hexanal + NADP(+) = (E)-hex--2-enal + H(+) + NADPH; Xref=Rhea:RHEA:50776, ChEBI:CHEBI:15378, ChEBI:CHEBI:28913, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:88528; Evidence={ECO:0000250\|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50778; Evidence={ECO:0000250\|UniProtKB:Q14914};
Catalytic activity:		Reaction=NADP(+) + octanal = (2E)-octenal + H(+) + NADPH; Xref=Rhea:RHEA:50780, ChEBI:CHEBI:15378, ChEBI:CHEBI:17935, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:61748; Evidence={ECO:0000250\|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50782; Evidence={ECO:0000250\|UniProtKB:Q14914};
Catalytic activity:		Reaction=decanal + NADP(+) = (2E)-decenal + H(+) + NADPH; Xref=Rhea:RHEA:50612, ChEBI:CHEBI:15378, ChEBI:CHEBI:31457, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133455; Evidence={ECO:0000250\|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50614; Evidence={ECO:0000250\|UniProtKB:Q14914};
Catalytic activity:		Reaction=dodecanal + NADP(+) = (2E)-dodecenal + H(+) + NADPH; Xref=Rhea:RHEA:50784, ChEBI:CHEBI:15378, ChEBI:CHEBI:27836, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:133741; Evidence={ECO:0000250\|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50786; Evidence={ECO:0000250\|UniProtKB:Q14914};
Catalytic activity:		Reaction=4-hydroxynonanal + NADP(+) = (E)-4-hydroxynon-2-enal + H(+) + NADPH; Xref=Rhea:RHEA:64736, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:58968, ChEBI:CHEBI:156112; Evidence={ECO:0000250\|UniProtKB:P97584}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:64738; Evidence={ECO:0000250\|UniProtKB:P97584};
Catalytic activity:		Reaction=NADP(+) + pentan-2-one = (E)-pent-3-en-2-one + H(+) + NADPH; Xref=Rhea:RHEA:50788, ChEBI:CHEBI:15378, ChEBI:CHEBI:16472, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:145276; Evidence={ECO:0000250\|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50790; Evidence={ECO:0000250\|UniProtKB:Q14914};
Catalytic activity:		Reaction=NADP(+) + nonan-2-one = (3E)-nonen-2-one + H(+) + NADPH; Xref=Rhea:RHEA:50616, ChEBI:CHEBI:15378, ChEBI:CHEBI:57783, ChEBI:CHEBI:58349, ChEBI:CHEBI:77927, ChEBI:CHEBI:133457; Evidence={ECO:0000250\|UniProtKB:Q14914}; PhysiologicalDirection=right-to-left; Xref=Rhea:RHEA:50618; Evidence={ECO:0000250\|UniProtKB:Q14914};
Biophysicochemical properties:		Kinetic parameters: KM=93 uM for LTB4 {ECO:0000269\|PubMed:11733004}; KM=35 uM for 15-keto-PGE2 {ECO:0000269\|PubMed:11733004}; Vmax=1.7 nmol/min/mg enzyme with LTB4 as substrate {ECO:0000269\|PubMed:11733004}; Vmax=345 nmol/min/mg enzyme with 15-keto-PGE2 as substrate {ECO:0000269\|PubMed:11733004};
Subunit:		Monomer or homodimer. {ECO:0000269\|PubMed:15007077}.
Subcellular location:		Cytoplasm {ECO:0000269\|PubMed:11733004}.
Tissue specificity:		Detected in small intestine, kidney, liver, spleen and stomach (at protein level). Detected in small intestine, kidney and liver. {ECO:0000269\|PubMed:11733004}.
Similarity:		Belongs to the NADP-dependent oxidoreductase L4BD family. {ECO:0000305}.