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UniProt functional annotation for Q53W83 | ||
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| UniProt code: Q53W83. |
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| Organism: | |
Thermus thermophilus (strain ATCC 27634 / DSM 579 / HB8). |
| Taxonomy: | |
Bacteria; Deinococcus-Thermus; Deinococci; Thermales; Thermaceae; Thermus. |
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| Function: | |
Involved in the degradation of glucose via the semi- phosphorylative Entner-Doudoroff pathway. Catalyzes the phosphorylation of 2-keto-3-deoxygluconate (KDG) to produce 2-keto-3-deoxy-6- phosphogluconate (KDPG). {ECO:0000269|PubMed:15210349}. |
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| Catalytic activity: | |
Reaction=2-dehydro-3-deoxy-D-gluconate + ATP = 2-dehydro-3-deoxy-6- phospho-D-gluconate + ADP + H(+); Xref=Rhea:RHEA:14797, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:57569, ChEBI:CHEBI:57990, ChEBI:CHEBI:456216; EC=2.7.1.45; Evidence={ECO:0000269|PubMed:15210349}; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=0.32 mM for KDG (at 35 degrees Celsius and pH 7.4) {ECO:0000269|PubMed:15210349}; KM=3.6 mM for 2-keto-D-gluconate (at 35 degrees Celsius and pH 7.4) {ECO:0000269|PubMed:15210349}; Note=Kcat is 11 (sec-1) for KDG and 2.2 (sec-1) for 2-keto-D- gluconate (at 35 degrees Celsius and pH 7.4).; pH dependence: Optimum pH is between 6 and 9. {ECO:0000269|PubMed:15210349}; |
| Pathway: | |
Carbohydrate acid metabolism; 2-dehydro-3-deoxy-D-gluconate degradation; D-glyceraldehyde 3-phosphate and pyruvate from 2-dehydro- 3-deoxy-D-gluconate: step 1/2. |
| Subunit: | |
Homohexamer; trimer of dimers. {ECO:0000269|PubMed:15210349}. |
| Similarity: | |
Belongs to the carbohydrate kinase pfkB family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.