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PDBsum entry 1uz3
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Chromatin regulator
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PDB id
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1uz3
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References listed in PDB file
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Key reference
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Title
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Crystal structure of the ent domain of human emsy.
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Authors
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G.B.Chavali,
C.M.Ekblad,
B.P.Basu,
N.C.Brissett,
D.Veprintsev,
L.Hughes-Davies,
T.Kouzarides,
L.S.Itzhaki,
A.J.Doherty.
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Ref.
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J Mol Biol, 2005,
350,
964-973.
[DOI no: ]
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PubMed id
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Abstract
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EMSY is a recently discovered gene encoding a BRCA2-associated protein and is
amplified in some sporadic breast and ovarian cancers. The EMSY sequence
contains no known domain except for a conserved approximately 100 residue
segment at the N terminus. This so-called ENT domain is unique in the human
genome, although multiple copies are found in Arabidopsis proteins containing
members of the Royal family of chromatin remodelling domains. Here, we report
the crystal structure of the ENT domain of EMSY, consisting of a unique
arrangement of five alpha-helices that fold into a helical bundle arrangement.
The fold shares regions of structural homology with the DNA-binding domain of
homeodomain proteins. The ENT domain forms a homodimer via the anti-parallel
packing of the extended N-terminal alpha-helix of each molecule. It is
stabilized mainly by hydrophobic residues at the dimer interface and has a
dissociation constant in the low micromolar range. The dimerisation of EMSY
mediated by the ENT domain could provide flexibility for it to bind two or more
different substrates simultaneously.
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Figure 3.
Figure 3. Structure of the ENT domain of EMSY. Crystal
structure of the ENT monomer showing the helical
bundle fold formed by the five helices in the molecule.
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Figure 4.
Figure 4. Structure of the homodimeric complex of ENT domains. (a) The secondary structure of EMSY1--100 showing
the two monomers A and B in pink and blue, respectively. The a-helices from chain A are denoted as a1-a5 and from
chain B as a10-a50. (b) The salt-bridges and hydrogen bonds, indicated by dotted lines, stabilizing the dimer visualized
using POVSCRIPTC.
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(c) Molecular surface representation of the ENT homodimer. A Coulomb method using only
charged residues was used to calculate the electrostatic potential, which was mapped to the molecular surface with red
representing negative charge and blue representing positive charge. The Figure was prepared using SwissPdb Viewer.
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The above figures are
reprinted
by permission from Elsevier:
J Mol Biol
(2005,
350,
964-973)
copyright 2005.
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