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UniProt functional annotation for Q39054 | ||
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| UniProt code: Q39054. |
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| Organism: | |
Arabidopsis thaliana (Mouse-ear cress). |
| Taxonomy: | |
Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta; Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae; rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis. |
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| Function: | |
Catalyzes two steps in the biosynthesis of the molybdenum cofactor. In the first step, molybdopterin is adenylated. Subsequently, molybdate is inserted into adenylated molybdopterin and AMP is released. {ECO:0000269|PubMed:12590921, ECO:0000269|PubMed:15306815, ECO:0000269|PubMed:15504727, ECO:0000269|PubMed:16636046}. |
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| Catalytic activity: | |
Reaction=adenylyl-molybdopterin + H(+) + molybdate = AMP + H2O + Mo- molybdopterin; Xref=Rhea:RHEA:35047, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:36264, ChEBI:CHEBI:62727, ChEBI:CHEBI:71302, ChEBI:CHEBI:456215; EC=2.10.1.1; |
| Catalytic activity: | |
Reaction=ATP + H(+) + molybdopterin = adenylyl-molybdopterin + diphosphate; Xref=Rhea:RHEA:31331, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:58698, ChEBI:CHEBI:62727; EC=2.7.7.75; |
| Cofactor: | |
Name=Mg(2+); Xref=ChEBI:CHEBI:18420; Evidence={ECO:0000269|PubMed:15306815, ECO:0000269|PubMed:15504727}; |
| Activity regulation: | |
Inhibited by copper and tungsten. {ECO:0000269|PubMed:15306815}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=62 uM for ATP for domain G {ECO:0000269|PubMed:15504727, ECO:0000269|PubMed:16636046}; KM=133 uM for Zn(2+) for domain E {ECO:0000269|PubMed:15504727, ECO:0000269|PubMed:16636046}; KM=255 uM for Mg(2+) for domain E {ECO:0000269|PubMed:15504727, ECO:0000269|PubMed:16636046}; |
| Pathway: | |
Cofactor biosynthesis; molybdopterin biosynthesis. |
| Subunit: | |
The G domain: homotrimer or homohexamer. The E domain: homodimer. {ECO:0000269|PubMed:11554796, ECO:0000269|PubMed:12590921, ECO:0000269|PubMed:15306815, ECO:0000269|PubMed:9341109}. |
| Similarity: | |
In the N-terminal section; belongs to the MoaB/Mog family. {ECO:0000305}. |
| Similarity: | |
In the C-terminal section; belongs to the MoeA family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.