UniProt functional annotation for P56658



	UniProt functional annotation for P56658

UniProt code: P56658.

Organism: Bos taurus (Bovine).

Taxonomy: Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.

Function: Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine (By similarity). Plays an important role in purine metabolism and in adenosine homeostasis (By similarity). Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events (By similarity). Acts as a positive regulator of T-cell coactivation, by binding DPP4 (By similarity). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (By similarity). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (PubMed:23240012). Enhances CD4+ T-cell differentiation and proliferation (By similarity). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (By similarity). Stimulates plasminogen activation (By similarity). Plays a role in male fertility (By similarity). Plays a protective role in early postimplantation embryonic development (By similarity). {ECO:0000250|UniProtKB:P00813, ECO:0000250|UniProtKB:P03958, ECO:0000269|PubMed:23240012}.

Catalytic activity: Reaction=adenosine + H(+) + H2O = inosine + NH4(+); Xref=Rhea:RHEA:24408, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16335, ChEBI:CHEBI:17596, ChEBI:CHEBI:28938; EC=3.5.4.4; Evidence={ECO:0000250|UniProtKB:P00813};

Cofactor: Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269|PubMed:12554940, ECO:0000269|PubMed:14709046, ECO:0000269|PubMed:15213224, ECO:0000269|PubMed:16033254}; Note=Binds 1 zinc ion per subunit. {ECO:0000269|PubMed:12554940};

Subunit: Interacts with DPP4 (via extracellular domain). Interacts with PLG (via Kringle 4 domain); the interaction stimulates PLG activation when in complex with DPP4. {ECO:0000250|UniProtKB:P00813}.

Subcellular location: Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Extracellular side {ECO:0000250}. Cell junction {ECO:0000250|UniProtKB:P00813}. Cytoplasmic vesicle lumen {ECO:0000250|UniProtKB:P03958}. Cytoplasm {ECO:0000250}. Lysosome {ECO:0000250|UniProtKB:P00813}. Note=Colocalized with DPP4 at the cell surface. {ECO:0000250|UniProtKB:P00813}.

Tissue specificity: Expressed in gastrointestinal tissues (at protein level). {ECO:0000269|PubMed:23240012}.

Mass spectrometry: Mass=40549; Mass_error=16; Method=Electrospray; Evidence={ECO:0000269|PubMed:8877857};

Pharmaceutical: Available under the name Adagen (Enzon). This is a PEG- conjugated form (pegademase). Used to treat patients with severe combined immunodeficiency diseases (SCID).

Similarity: Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. {ECO:0000305}.

Annotations taken from UniProtKB at the EBI.


Organism:		Bos taurus (Bovine).
Taxonomy:		Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae; Bovinae; Bos.



Function:		Catalyzes the hydrolytic deamination of adenosine and 2- deoxyadenosine (By similarity). Plays an important role in purine metabolism and in adenosine homeostasis (By similarity). Modulates signaling by extracellular adenosine, and so contributes indirectly to cellular signaling events (By similarity). Acts as a positive regulator of T-cell coactivation, by binding DPP4 (By similarity). Its interaction with DPP4 regulates lymphocyte-epithelial cell adhesion (By similarity). Enhances dendritic cell immunogenicity by affecting dendritic cell costimulatory molecule expression and cytokines and chemokines secretion (PubMed:23240012). Enhances CD4+ T-cell differentiation and proliferation (By similarity). Acts as a positive modulator of adenosine receptors ADORA1 and ADORA2A, by enhancing their ligand affinity via conformational change (By similarity). Stimulates plasminogen activation (By similarity). Plays a role in male fertility (By similarity). Plays a protective role in early postimplantation embryonic development (By similarity). {ECO:0000250\|UniProtKB:P00813, ECO:0000250\|UniProtKB:P03958, ECO:0000269\|PubMed:23240012}.


Catalytic activity:		Reaction=adenosine + H(+) + H2O = inosine + NH4(+); Xref=Rhea:RHEA:24408, ChEBI:CHEBI:15377, ChEBI:CHEBI:15378, ChEBI:CHEBI:16335, ChEBI:CHEBI:17596, ChEBI:CHEBI:28938; EC=3.5.4.4; Evidence={ECO:0000250\|UniProtKB:P00813};
Cofactor:		Name=Zn(2+); Xref=ChEBI:CHEBI:29105; Evidence={ECO:0000269\|PubMed:12554940, ECO:0000269\|PubMed:14709046, ECO:0000269\|PubMed:15213224, ECO:0000269\|PubMed:16033254}; Note=Binds 1 zinc ion per subunit. {ECO:0000269\|PubMed:12554940};
Subunit:		Interacts with DPP4 (via extracellular domain). Interacts with PLG (via Kringle 4 domain); the interaction stimulates PLG activation when in complex with DPP4. {ECO:0000250\|UniProtKB:P00813}.
Subcellular location:		Cell membrane {ECO:0000250}; Peripheral membrane protein {ECO:0000250}; Extracellular side {ECO:0000250}. Cell junction {ECO:0000250\|UniProtKB:P00813}. Cytoplasmic vesicle lumen {ECO:0000250\|UniProtKB:P03958}. Cytoplasm {ECO:0000250}. Lysosome {ECO:0000250\|UniProtKB:P00813}. Note=Colocalized with DPP4 at the cell surface. {ECO:0000250\|UniProtKB:P00813}.
Tissue specificity:		Expressed in gastrointestinal tissues (at protein level). {ECO:0000269\|PubMed:23240012}.
Mass spectrometry:		Mass=40549; Mass_error=16; Method=Electrospray; Evidence={ECO:0000269\|PubMed:8877857};
Pharmaceutical:		Available under the name Adagen (Enzon). This is a PEG- conjugated form (pegademase). Used to treat patients with severe combined immunodeficiency diseases (SCID).
Similarity:		Belongs to the metallo-dependent hydrolases superfamily. Adenosine and AMP deaminases family. {ECO:0000305}.