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UniProt functional annotation for P62617 | ||
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| UniProt code: P62617. |
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| Organism: | |
Escherichia coli (strain K12). |
| Taxonomy: | |
Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Escherichia. |
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| Function: | |
Involved in the biosynthesis of isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP), two major building blocks of isoprenoid compounds. Catalyzes the conversion of 4-diphosphocytidyl-2- C-methyl-D-erythritol 2-phosphate (CDP-ME2P) to 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (ME-CPP) with a corresponding release of cytidine 5-monophosphate (CMP). Also converts 4-diphosphocytidyl-2-C-methyl-D- erythritol into 2-C-methyl-D-erythritol 3,4-cyclophosphate and CMP. {ECO:0000269|PubMed:10694574, ECO:0000269|PubMed:22839733}. |
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| Catalytic activity: | |
Reaction=4-CDP-2-C-methyl-D-erythritol 2-phosphate = 2-C-methyl-D- erythritol 2,4-cyclic diphosphate + CMP; Xref=Rhea:RHEA:23864, ChEBI:CHEBI:57919, ChEBI:CHEBI:58483, ChEBI:CHEBI:60377; EC=4.6.1.12; Evidence={ECO:0000269|PubMed:10694574, ECO:0000269|PubMed:22839733}; |
| Cofactor: | |
Name=a divalent metal cation; Xref=ChEBI:CHEBI:60240; Evidence={ECO:0000269|PubMed:10694574, ECO:0000269|PubMed:11786530, ECO:0000269|PubMed:11829504, ECO:0000269|PubMed:11997478, ECO:0000269|PubMed:15608374, ECO:0000269|PubMed:16392111, ECO:0000269|PubMed:16511114, ECO:0000269|PubMed:19320487}; Note=Binds 1 divalent metal cation per subunit. {ECO:0000269|PubMed:10694574, ECO:0000269|PubMed:11786530, ECO:0000269|PubMed:11829504, ECO:0000269|PubMed:11997478, ECO:0000269|PubMed:15608374, ECO:0000269|PubMed:16392111, ECO:0000269|PubMed:16511114, ECO:0000269|PubMed:19320487}; |
| Activity regulation: | |
Activated by 2C-methyl-D-erythritol 4-phosphate (MEP). {ECO:0000269|PubMed:22839733}. |
| Biophysicochemical properties: | |
Kinetic parameters: KM=339 uM for CDP-ME2P (at pH 7.4) {ECO:0000269|PubMed:22839733}; Note=kcat is 61 min(-1) for CDP-ME2P (at pH 7.4).; |
| Pathway: | |
Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5- phosphate: step 4/6. |
| Subunit: | |
Homotrimer. {ECO:0000269|PubMed:11786530, ECO:0000269|PubMed:11829504, ECO:0000269|PubMed:11997478, ECO:0000269|PubMed:15608374, ECO:0000269|PubMed:16392111, ECO:0000269|PubMed:16511114, ECO:0000269|PubMed:19320487}. |
| Disruption phenotype: | |
Cells lacking this gene reveal a filamentous phenotype. {ECO:0000269|PubMed:12270818}. |
| Similarity: | |
Belongs to the IspF family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.