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UniProt functional annotation for Q9X0C6 | ||
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| UniProt code: Q9X0C6. |
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| Organism: | |
Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8). |
| Taxonomy: | |
Bacteria; Thermotogae; Thermotogales; Thermotogaceae; Thermotoga. |
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| Function: | |
IGPS catalyzes the conversion of PRFAR and glutamine to IGP, AICAR and glutamate. The HisF subunit catalyzes the cyclization activity that produces IGP and AICAR from PRFAR using the ammonia provided by the HisH subunit. |
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| Catalytic activity: | |
Reaction=5-[(5-phospho-1-deoxy-D-ribulos-1-ylimino)methylamino]-1-(5- phospho-beta-D-ribosyl)imidazole-4-carboxamide + L-glutamine = 5- amino-1-(5-phospho-beta-D-ribosyl)imidazole-4-carboxamide + D- erythro-1-(imidazol-4-yl)glycerol 3-phosphate + H(+) + L-glutamate; Xref=Rhea:RHEA:24793, ChEBI:CHEBI:15378, ChEBI:CHEBI:29985, ChEBI:CHEBI:58278, ChEBI:CHEBI:58359, ChEBI:CHEBI:58475, ChEBI:CHEBI:58525; EC=4.3.2.10; |
| Biophysicochemical properties: | |
Kinetic parameters: KM=1.5 uM for PRFAR {ECO:0000269|PubMed:11264293}; KM=2.2 mM for NH(3) {ECO:0000269|PubMed:11264293}; |
| Pathway: | |
Amino-acid biosynthesis; L-histidine biosynthesis; L-histidine from 5-phospho-alpha-D-ribose 1-diphosphate: step 5/9. |
| Subunit: | |
Heterodimer of HisH and HisF. |
| Subcellular location: | |
Cytoplasm. |
| Similarity: | |
Belongs to the HisA/HisF family. {ECO:0000305}. |
Annotations taken from UniProtKB at the EBI.